Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human LAG1 longevity assurance homolog 6 (LASS6) Standard Curve.)
Human LAG1 Longevity Assurance Homolog 6 (LASS6) ELISA Kit | LASS6 elisa kit
Human LAG1 Longevity Assurance Homolog 6 (LASS6) ELISA Kit
Gene Names
CERS6; CERS5; LASS6
Reactivity
Human
Synonyms
LAG1 Longevity Assurance Homolog 6 (LASS6); Human LAG1 Longevity Assurance Homolog 6 (LASS6) ELISA Kit; LASS6; CerS6; MGC129949; MGC129950; LAG1 longevity assurance homolog 6; longevity assurance homolog 6; LASS6 elisa kit
Reactivity
Human
Specificity
Human LAG1 longevity assurance homolog 6 (LASS6) ELISA Kit has high sensitivity and excellent specificity for detection of Human LASS6. No significant cross-reactivity or interference between Human LASS6 and analogues was observed.
Sequence Length
392
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human LAG1 longevity assurance homolog 6 (LASS6) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human LAG1 longevity assurance homolog 6 (LASS6) Standard Curve.)
Related Product Information for LASS6 elisa kit
Description: This Human LAG1 longevity assurance homolog 6 (LASS6) ELISA Kit employs a two-site sandwich ELISA to quantitate LASS6 in samples. An antibody specific for LASS6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LASS6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LASS6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LASS6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Longevity assurance genes (LAGs) represent a subgroup of the homeobox gene family.Like other LAG family members, the LASS6 protein contained a homeodomain and LAG1 domain. In phylogenetic analyses, it displayed highest homology to LASS5. The corresponding gene was localized to human chromosome 2q24.3, spanning a rather large genomic region of 318 kb. Orthologous sequences in mouse and zebrafish suggested a conservation of LASS6 in vertebrates as the protein and corresponding genomic sequences were highly conserved. LASS6 expression was analyzed in silico, and the gene was shown to be broadly expressed in a wide range of tissues. Furthermore, available microarray data suggested a role in cancer differentiation and early embryonic development.
Background: Longevity assurance genes (LAGs) represent a subgroup of the homeobox gene family.Like other LAG family members, the LASS6 protein contained a homeodomain and LAG1 domain. In phylogenetic analyses, it displayed highest homology to LASS5. The corresponding gene was localized to human chromosome 2q24.3, spanning a rather large genomic region of 318 kb. Orthologous sequences in mouse and zebrafish suggested a conservation of LASS6 in vertebrates as the protein and corresponding genomic sequences were highly conserved. LASS6 expression was analyzed in silico, and the gene was shown to be broadly expressed in a wide range of tissues. Furthermore, available microarray data suggested a role in cancer differentiation and early embryonic development.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
LASS6 protein
NCBI Official Synonym Full Names
ceramide synthase 6
NCBI Official Symbol
CERS6
NCBI Official Synonym Symbols
CERS5; LASS6
NCBI Protein Information
ceramide synthase 6
UniProt Protein Name
Ceramide synthase 6
UniProt Gene Name
CERS6
UniProt Synonym Gene Names
LASS6; CerS6
UniProt Entry Name
CERS6_HUMAN
Uniprot Description
LASS6: May be involved in sphingolipid synthesis or its regulation.
Protein type: DNA-binding; Membrane protein, integral; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 2q24.3
Cellular Component: endoplasmic reticulum membrane; nuclear membrane; membrane; endoplasmic reticulum; integral to membrane
Molecular Function: DNA binding; sphingosine N-acyltransferase activity
Biological Process: sphingolipid metabolic process; sphingolipid biosynthetic process; ceramide biosynthetic process
Research Articles on LASS6
Similar Products
Product Notes
The Human LASS6 cers6 (Catalog #AAA9715516) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715516 ELISA Kit recognizes Human LASS6. It is sometimes possible for the material contained within the vial of "LAG1 Longevity Assurance Homolog 6 (LASS6), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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