Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human LAG1 longevity assurance homolog 2 (LASS2) Standard Curve.)
Human LAG1 Longevity Assurance Homolog 2 (LASS2) ELISA Kit | LASS2 elisa kit
Human LAG1 Longevity Assurance Homolog 2 (LASS2) ELISA Kit
Gene Names
CERS2; L3; LASS2; SP260; TMSG1
Reactivity
Human
Synonyms
LAG1 Longevity Assurance Homolog 2 (LASS2); Human LAG1 Longevity Assurance Homolog 2 (LASS2) ELISA Kit; LASS2; CerS2; FLJ10243; L3; MGC987; SP260; TMSG1; LAG1 longevity assurance 2; ceramide synthase 2; longevity assurance (LAG1; S. cerevisiae) homolog 2; tumor metastasis-suppressor; LASS2 elisa kit
Reactivity
Human
Specificity
Human LAG1 longevity assurance homolog 2 (LASS2) ELISA Kit has high sensitivity and excellent specificity for detection of Human LASS2. No significant cross-reactivity or interference between Human LASS2 and analogues was observed.
Sequence Length
380
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human LAG1 longevity assurance homolog 2 (LASS2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human LAG1 longevity assurance homolog 2 (LASS2) Standard Curve.)
Related Product Information for LASS2 elisa kit
Description: This Human LAG1 longevity assurance homolog 2 (LASS2) ELISA Kit employs a two-site sandwich ELISA to quantitate LASS2 in samples. An antibody specific for LASS2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LASS2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LASS2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LASS2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: LASS2 has sequence similarity to yeast longevity assurance gene 1. Mutation or overexpression of the related gene in yeast has been shown to alter yeast lifespan. The human protein may play a role in the regulation of cell growth. Alternatively spliced transcript variants encoding the same protein have been described. The deduced 230-amino acid protein is 29% identical to LASS1 over 224 amino acids. It has 4 transmembrane helices (2 fewer than most Lag1 homologs), a Lag1 motif, and a C-terminal acidic domain. Northern blot analysis revealed expression of a 2.4-kb transcript in kidney and liver, with lower levels in brain, heart, placenta, and lung.Expression of LASS2 in hepatocellular carcinoma cell lines led to suppression of cancer cell growth.
Background: LASS2 has sequence similarity to yeast longevity assurance gene 1. Mutation or overexpression of the related gene in yeast has been shown to alter yeast lifespan. The human protein may play a role in the regulation of cell growth. Alternatively spliced transcript variants encoding the same protein have been described. The deduced 230-amino acid protein is 29% identical to LASS1 over 224 amino acids. It has 4 transmembrane helices (2 fewer than most Lag1 homologs), a Lag1 motif, and a C-terminal acidic domain. Northern blot analysis revealed expression of a 2.4-kb transcript in kidney and liver, with lower levels in brain, heart, placenta, and lung.Expression of LASS2 in hepatocellular carcinoma cell lines led to suppression of cancer cell growth.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
LAG1 longevity assurance homolog 2 (S. cerevisiae), isoform CRA_a
NCBI Official Synonym Full Names
ceramide synthase 2
NCBI Official Symbol
CERS2
NCBI Official Synonym Symbols
L3; LASS2; SP260; TMSG1
NCBI Protein Information
ceramide synthase 2
UniProt Protein Name
Ceramide synthase 2
UniProt Gene Name
CERS2
UniProt Synonym Gene Names
LASS2; TMSG1; CerS2
UniProt Entry Name
CERS2_HUMAN
NCBI Description
This gene encodes a protein that has sequence similarity to yeast longevity assurance gene 1. Mutation or overexpression of the related gene in yeast has been shown to alter yeast lifespan. The human protein may play a role in the regulation of cell growth. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008]
Uniprot Description
Function: Suppresses the growth of cancer cells. May be involved in sphingolipid synthesis.
Subunit structure: Interacts with ATP6V0C, ASGR1, ASGR2 and SLC22A1/OCT1. Interacts with ELOV1, HSD17B12 and TECR. Ref.1 Ref.15
Subcellular location: Nucleus membrane; Multi-pass membrane protein
Potential. Endoplasmic reticulum membrane; Multi-pass membrane protein
By similarity.
Tissue specificity: Expressed in kidney, liver, brain, heart, placenta and lung. Ref.1
Sequence similarities: Contains 1 homeobox DNA-binding domain.Contains 1 TLC (TRAM/LAG1/CLN8) domain.
Sequence caution: The sequence BAA91505.1 differs from that shown. Reason: Erroneous initiation.
Research Articles on LASS2
Similar Products
Product Notes
The Human LASS2 cers2 (Catalog #AAA9715517) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715517 ELISA Kit recognizes Human LASS2. It is sometimes possible for the material contained within the vial of "LAG1 Longevity Assurance Homolog 2 (LASS2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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