Rabbit anti-Human, Mouse AHR Polyclonal Antibody | anti-AHR antibody

AHR, ID (AHR, BHLHE76, Aryl hydrocarbon receptor, Class E basic helix-loop-helix protein 76) (Biotin)

Cat. Num. AAA6272357

• Gene Names: AHR; bHLHe76
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay, ELISA
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: AHR; Polyclonal Antibody; ID (AHR; BHLHE76; Aryl hydrocarbon receptor; Class E basic helix-loop-helix protein 76) (Biotin); Class E basic helix-loop-helix protein 76; anti-AHR antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human AHR. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-AHR antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 555-582 in the central region of human AHR.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of mouse NIH-3T3 cell (lane 1) and T47D cell (lane 2) lysates (35ug/lane) using antibodyMBS6003829.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human lung carcinoma using antibodyMBS6003829. Peroxidase-conjugated secondary antibody and DAB staining were used for visualization of the antigen.)

Immunofluorescence (IF)

(Confocal IF analysis of HeLa cells using antibody MBS6003829 and Alexa Fluor488-conjugated goat anti-rabbit secondary antibody (green). DAPI was used to stain the cell nuclei (blue).)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of NCI-H460 cells using MBS6003829 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibody was used for the analysis.)

Product Categories/Family for anti-AHR antibody

Antibodies; Receptors

References

1. Davila, S., et al. Genes Immun. (2010). 2. Kalthoff, S., et al. J. Biol. Chem. 285(9):5993-6002(2010). 3. Hall, J.M., et al. Mol. Endocrinol. 24(2):359-369(2010). 4. Schroeder, J.C., et al. Biochemistry 49(2):393-400(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 4502003
• NCBI GeneID: 196
• NCBI Accession #: NP_001612.1
• NCBI GenBank Nucleotide #: NM_001621.4
• UniProt Accession #: P35869; Q13728; Q13803; Q13804; A4D130
• Molecular Weight: 96,147 Da
• NCBI Official Full Name: aryl hydrocarbon receptor
• NCBI Official Synonym Full Names: aryl hydrocarbon receptor
• NCBI Official Symbol: AHR
• NCBI Official Synonym Symbols: bHLHe76
• NCBI Protein Information: aryl hydrocarbon receptor; AH-receptor; ah receptor; aromatic hydrocarbon receptor; class E basic helix-loop-helix protein 76
• UniProt Protein Name: Aryl hydrocarbon receptor
• Protein Family: Aldehyde reductase
• UniProt Gene Name: AHR
• UniProt Synonym Gene Names: BHLHE76; Ah receptor; AhR; bHLHe76
• UniProt Entry Name: AHR_HUMAN

NCBI Description

This gene encodes a ligand-activated transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. This receptor has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450. Its ligands included a variety of aromatic hydrocarbons. [provided by RefSeq, Jul 2008]

Uniprot Description

AHR: a nuclear receptor for aryl hydrocarbons involved in xenobiotic metabolism, cell cycle regulation, and development in response to both endogenous and environmental signals. AhR was initially identified as a receptor for dioxins, which are environmental pollutants generated by waste incineration and other industrial processes . AhR ligands include polycyclic aromatic hydrocarbons, including the carcinogen benzo(a)pyrene and other components of cigarette smoke. Naturally occurring AhR ligands include flavonoids, which are aromatic plant secondary compounds commonly found in vegetables and fruits. Cytoplasmic aryl hydrocarbon receptors are found in protein complexes with heat shock proteins. Upon ligand binding, AhR dissociates from heat shock proteins and translocate to the nucleus where it dimerizes with AhR nuclear translocator (ARNT, HIF-1b). The AhR/ARNT heterodimer binds to nuclear xenobiotic response elements to control the expression of genes associated with xenobiotic metabolism, including several cytochrome P450 genes. AhR is ubiquitously expressed and is thought to play a role in regulation of cell proliferation and differentiation, cytokine expression, and xenobiotic metabolism. Research studies link AhR activity with the control of regulatory T-cell and T-helper 17 cell differentiation, regulation of the inflammatory response, and the onset of lung cancer.

Protein type: DNA-binding; Transcription factor; Nuclear receptor

Chromosomal Location of Human Ortholog: 7p15

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein dimerization activity; signal transducer activity; protein binding; ligand-dependent nuclear receptor activity; DNA binding; sequence-specific DNA binding; protein heterodimerization activity; Hsp90 protein binding; transcription factor activity; transcription factor binding

Biological Process: transcription from RNA polymerase II promoter; prostate gland development; blood vessel development; intracellular receptor-mediated signaling pathway; apoptosis; response to toxin; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; cell cycle; regulation of transcription from RNA polymerase II promoter; response to xenobiotic stimulus; regulation of transcription, DNA-dependent; regulation of gene expression; xenobiotic metabolic process; positive regulation of transcription from RNA polymerase II promoter; regulation of B cell proliferation; circadian regulation of gene expression; positive regulation of transcriptional preinitiation complex assembly; negative regulation of transcription, DNA-dependent