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Typical Testing Data/Standard Curve (for reference only)

Human GFRA3 ELISA Kit | GFRA3 elisa kit

Human GFRA3 PicoKine ELISA Kit

Gene Names
GFRA3; GDNFR3
Reactivity
Human
Synonyms
GFRA3; Human GFRA3 PicoKine ELISA Kit; Human GFRA3; GDNF family receptor alpha 3; GDNF family receptor alpha-3; GDNF receptor alpha-3; GDNFR-alpha-3; GFR-alpha-3; UNQ339; PRO538; PRO3664; GFRA3 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
Natural and recombinant Human GFRA3
Sequence Length
400
Samples
Cell culture supernates, serum and plasma (heparin, EDTA, citrate)
Detection Range
46.9pg/ml-3000pg/ml
Sensitivity
<20pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for GFRA3 elisa kit
Principle of the Assay: The Picokine™ Human GFRA3 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human GFRA3 with a 96-well strip plate that is pre-coated with antibody specific for GFRA3. The detection antibody is a biotinylated antibody specific for GFRA3. The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Human GFRA3 with immunogen: Expression system for standard: NSO, Immunogen sequence: D32-N374. The kit is analytically validated with ready to use reagents. To measure Human GFRA3, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human GFRA3 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human GFRA3 in the sample.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
GDNF family receptor alpha-3
NCBI Official Synonym Full Names
GDNF family receptor alpha 3
NCBI Official Symbol
GFRA3
NCBI Official Synonym Symbols
GDNFR3
NCBI Protein Information
GDNF family receptor alpha-3
UniProt Protein Name
GDNF family receptor alpha-3
Protein Family
UniProt Gene Name
GFRA3
UniProt Synonym Gene Names
GDNF receptor alpha-3; GDNFR-alpha-3; GFR-alpha-3
UniProt Entry Name
GFRA3_HUMAN

NCBI Description

The protein encoded by this gene is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor and a member of the GDNF receptor family. It forms a signaling receptor complex with RET tyrosine kinase receptor and binds the ligand, artemin. [provided by RefSeq, Jul 2008]

Uniprot Description

GFRA3: Receptor for the glial cell line-derived neurotrophic factor, ARTN (artemin). Mediates the artemin-induced autophosphorylation and activation of the RET receptor tyrosine kinase. Belongs to the GDNFR family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 5q31.1-q31.3

Cellular Component: extrinsic to membrane; intracellular membrane-bound organelle; cytoplasm; plasma membrane; external side of plasma membrane

Molecular Function: axon guidance receptor activity; receptor binding

Biological Process: axon guidance; nervous system development; sympathetic nervous system development; neuron migration; signal transduction; peripheral nervous system development

Research Articles on GFRA3

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Product Notes

The Human GFRA3 gfra3 (Catalog #AAA1752680) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1752680 ELISA Kit recognizes Human GFRA3. It is sometimes possible for the material contained within the vial of "GFRA3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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