Mouse anti-Human PP2Ac / PPP2CA Monoclonal Antibody | anti-PP2Ac antibody

Mouse Monoclonal [clone P6C7] (IgG2b,k) to Human PP2Ac / PPP2CA

Cat. Num. AAA245334

• Gene Names: PPP2CA; RP-C; PP2Ac; PP2CA; PP2Calpha
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Immunoprecipitation, ELISA
• Purity: Protein G Purified
• Synonyms: PP2Ac / PPP2CA; Monoclonal Antibody; Mouse Monoclonal [clone P6C7] (IgG2b; k) to Human PP2Ac / PPP2CA; Anti-PP2Ac / PPP2CA Antibody (clone P6C7) IHC-plus; PPP2CA; PP2Ac; PP2Calpha; ppp2A1; Protein phosphatase 2A alpha; Replication protein C; PP2CA; RP-C; PP2A-alpha; Human PP2Ac; anti-PP2Ac antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone Number: P6C7
• Purity/Purification: Protein G Purified
• Form/Format: PBS, pH 7.4, 0.1% sodium azide
• Sequence Length: 309

• Applicable Applications for anti-PP2Ac antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Immunoprecipitation (IP), ELISA (EIA)
• Application Notes: ELISA, IHC-P (15 ug/ml), IP, WB (1:250 - 1:1000); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Recombinant human PP2Ckappa protein.
• Immunogen Type: Recombinant protein
• Immunogen: PP2Ac / PPP2CA antibody was raised against recombinant human PP2Ckappa protein.
• Preparation and Storage: Long term: -20 degree C; Short term: +4 degree C; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-PPP2CA / PP2Ac antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 15 ug/ml.)

Western Blot (WB)

(Mouse liver cytosol extract was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-PP2Ca(1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a DAP detection system. Arrow indicates PP2Ca(~43kD).)

Related Product Information for anti-PP2Ac antibody

Protein phosphatase 2A alpha (PPP2CA), is a PP2A type serine/threonine phosphatase. PP2A members are composed of a common heterodimeric core enzyme (a catalytic subunit C and a constant regulatory subunit PR65 or A) that associates with a wide variety of regulatory subunits (subunit B). PPP2CA is a catalytic subunit that plays a role in transcription, translation, cell cycle control, and the regulation of most major metabolic pathways.

Product Categories/Family for anti-PP2Ac antibody

Family: Protein Phosphatase; Subfamily: PP2A

NCBI and Uniprot Product Information

• NCBI GI #: 4506017
• NCBI GeneID: 5515
• NCBI Accession #: NP_002706.1
• NCBI GenBank Nucleotide #: NM_002715.2
• UniProt Accession #: P67775; P05323; P13197
• Molecular Weight: 29,735 Da
• NCBI Official Full Name: serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
• NCBI Official Synonym Full Names: protein phosphatase 2, catalytic subunit, alpha isozyme
• NCBI Official Symbol: PPP2CA
• NCBI Official Synonym Symbols: RP-C; PP2Ac; PP2CA; PP2Calpha
• NCBI Protein Information: serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform; PP2A-alpha; replication protein C; protein phosphatase 2A catalytic subunit, alpha isoform; protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform; serine/threonine protein phosphatase 2A, catalytic subunit, alpha isoform
• UniProt Protein Name: Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
• UniProt Gene Name: PPP2CA
• UniProt Synonym Gene Names: PP2A-alpha; RP-C
• UniProt Entry Name: PP2AA_HUMAN

NCBI Description

This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. [provided by RefSeq, Jul 2008]

Uniprot Description

PPP2CA: ubiquitous cytosolic Ser/Thr protein phosphatase, catalytic subunit. The holoenzyme consists of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B'/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules.

Protein type: Motility/polarity/chemotaxis; EC 3.1.3.16; Protein phosphatase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: microtubule cytoskeleton; spindle pole; protein phosphatase type 2A complex; mitochondrion; membrane; plasma membrane; cytosol; nucleus; chromosome, pericentric region

Molecular Function: protein dimerization activity; protein C-terminus binding; protein binding; metal ion binding; GABA receptor binding; protein serine/threonine phosphatase activity

Biological Process: second-messenger-mediated signaling; regulation of cell adhesion; mitotic nuclear envelope reassembly; fibroblast growth factor receptor signaling pathway; apoptosis; RNA splicing; regulation of protein amino acid autophosphorylation; regulation of Wnt receptor signaling pathway; protein amino acid dephosphorylation; response to organic substance; regulation of transcription, DNA-dependent; meiotic cell cycle; regulation of cell differentiation; regulation of growth; negative regulation of tyrosine phosphorylation of Stat3 protein; mRNA catabolic process, nonsense-mediated decay; mesoderm development; regulation of protein catabolic process; gene expression; mitotic cell cycle; regulation of receptor activity; negative regulation of cell growth; ceramide metabolic process; regulation of DNA replication; inactivation of MAPK activity