Typical Testing Data/Standard Curve (for reference only)
Human SPOCK1/Testican-1 ELISA Kit | SPOCK1 elisa kit
Human SPOCK1/Testican-1 PicoKine ELISA Kit
Gene Names
SPOCK1; TIC1; SPOCK; TESTICAN
Reactivity
Human
Synonyms
SPOCK1/Testican-1; Human SPOCK1/Testican-1 PicoKine ELISA Kit; Human SPOCK1/Testican-1; SPARC (osteonectin); cwcv and kazal like domains proteoglycan 1; Testican-1; Protein SPOCK; SPOCK1; SPOCK; TIC1; TICN1; SPOCK1 elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human SPOCK1
Sequence Length
439
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Detection Range
15.6pg/ml-1000pg/ml
Sensitivity
<10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for SPOCK1 elisa kit
Principle of the Assay: The Picokine™ Human SPOCK1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human SPOCK1 with a 96-well strip plate that is pre-coated with antibody specific for SPOCK1. The detection antibody is a biotinylated antibody specific for SPOCK1. The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Human SPOCK1 with immunogen: Expression system for standard: NSO, Immunogen sequence: R22-W439. The kit is analytically validated with ready to use reagents. To measure Human SPOCK1, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human SPOCK1 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human SPOCK1 in the sample.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
testican-1
NCBI Official Synonym Full Names
SPARC (osteonectin), cwcv and kazal like domains proteoglycan 1
NCBI Official Symbol
SPOCK1
NCBI Official Synonym Symbols
TIC1; SPOCK; TESTICAN
NCBI Protein Information
testican-1
UniProt Protein Name
Testican-1
Protein Family
UniProt Gene Name
SPOCK1
UniProt Synonym Gene Names
SPOCK; TIC1; TICN1
NCBI Description
This gene encodes the protein core of a seminal plasma proteoglycan containing chondroitin- and heparan-sulfate chains. The protein's function is unknown, although similarity to thyropin-type cysteine protease-inhibitors suggests its function may be related to protease inhibition. [provided by RefSeq, Jul 2008]
Uniprot Description
SPOCK1: May play a role in cell-cell and cell-matrix interactions. May contribute to various neuronal mechanisms in the central nervous system.
Protein type: Cell adhesion; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 5q31.2
Cellular Component: cytoplasm; extracellular space; neuromuscular junction; sarcoplasm
Molecular Function: calcium ion binding; cysteine protease inhibitor activity; metalloendopeptidase inhibitor activity
Biological Process: central nervous system neuron differentiation; neurogenesis; neuron migration
Research Articles on SPOCK1
Similar Products
Product Notes
The Human SPOCK1 spock1 (Catalog #AAA1752579) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1752579 ELISA Kit recognizes Human SPOCK1. It is sometimes possible for the material contained within the vial of "SPOCK1/Testican-1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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