Human CD99L2 ELISA Kit | CD99L2 elisa kit

Human CD99L2 PicoKine ELISA Kit

Cat. Num. AAA1752695

• Gene Names: CD99L2; CD99B; MIC2L1
• Reactivity: Human
• Synonyms: CD99L2; Human CD99L2 PicoKine ELISA Kit; Human CD99L2; CD99 molecule like 2; CD99 antigen-like protein 2; MIC2-like protein 1; CD99; MIC2L1; UNQ1964; PRO4486; CD99L2 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human CD99L2
• Sequence Length: 189

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA, citrate)
• Detection Range: 312pg/ml-20000pg/ml
• Sensitivity: <50pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CD99L2 elisa kit

Principle of the Assay: The Picokine™ Human CD99L2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human CD99L2 with a 96-well strip plate that is pre-coated with antibody specific for CD99L2. The detection antibody is a biotinylated antibody specific for CD99L2. The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Human CD99L2 with immunogen: Expression system for standard: E.coli, Immunogen sequence: D26-G185. The kit is analytically validated with ready to use reagents. To measure Human CD99L2, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human CD99L2 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CD99L2 in the sample.!!Background/Introduction: CD99 antigen-like protein 2 is a protein that in humans is encoded by the CD99L2 gene. It is mapped to Xq28. This gene encodes a cell-surface protein that is similar to CD99. A similar protein in mouse functions as an adhesion molecule during leukocyte extravasation. Alternate splicing results in multiple transcript variants.

NCBI and Uniprot Product Information

• NCBI GI #: 296317276
• NCBI GeneID: 83692
• NCBI Accession #: NP_001171737.1
• NCBI GenBank Nucleotide #: NP_001171737.1
• UniProt Accession #: Q8TCZ2
• NCBI Official Full Name: CD99 antigen-like protein 2 isoform 4
• NCBI Official Synonym Full Names: CD99 molecule like 2
• NCBI Official Symbol: CD99L2
• NCBI Official Synonym Symbols: CD99B; MIC2L1
• NCBI Protein Information: CD99 antigen-like protein 2
• UniProt Protein Name: CD99 antigen-like protein 2
• Protein Family: CD99 antigen-like protein
• UniProt Gene Name: CD99L2
• UniProt Synonym Gene Names: MIC2L1; UNQ1964/PRO4486
• UniProt Entry Name: C99L2_HUMAN

NCBI Description

This gene encodes a cell-surface protein that is similar to CD99. A similar protein in mouse functions as an adhesion molecule during leukocyte extravasation. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2010]

Uniprot Description

CD99L2: Plays a role in a late step of leukocyte extravasation helping cells to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Homophilic adhesion molecule, but these interactions may not be required for cell aggregation. Belongs to the CD99 family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: focal adhesion; integral to membrane; plasma membrane

Biological Process: cell adhesion