Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Typical Testing Data/Standard Curve (for reference only)

Mouse GFRA2 ELISA Kit | GFRA2 elisa kit

Mouse GFRA2 PicoKine ELISA Kit

Reactivity
Mouse
Synonyms
GFRA2; Mouse GFRA2 PicoKine ELISA Kit; Mouse GFRA2; glial cell line derived neurotrophic factor family receptor alpha 2; GDNF family receptor alpha-2; GDNF receptor alpha-2; GDNFR-alpha-2; GFR-alpha-2; GDNF receptor beta; GDNFR-beta; Neurturin receptor alpha; NRTNR-alpha; NTNR-alpha; TGF-beta-related neurotrophic factor receptor 2; Gfra2; Gdnfrb; Trnr2; GFRA2 elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Specificity
Natural and recombinant Mouse Gfra2
Sequence Length
463
Samples
Cell culture supernates, serum and plasma (heparin, EDTA, citrate)
Detection Range
312pg/ml-20000pg/ml
Sensitivity
<50pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for GFRA2 elisa kit
Principle of the Assay: The Picokine™ Mouse Gfra2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse Gfra2 with a 96-well strip plate that is pre-coated with antibody specific for Gfra2. The detection antibody is a biotinylated antibody specific for Gfra2. The capture antibody is a monoclonal antibody from rat, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Mouse Gfra2 with immunogen: Expression system for standard: NSO, Immunogen sequence: S22-S443. The kit is analytically validated with ready to use reagents. To measure Mouse Gfra2, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Mouse Gfra2 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Gfra2 in the sample.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
GDNF family receptor alpha-2
NCBI Official Synonym Full Names
glial cell line derived neurotrophic factor family receptor alpha 2
NCBI Official Symbol
Gfra2
NCBI Protein Information
GDNF family receptor alpha-2
UniProt Protein Name
GDNF family receptor alpha-2
Protein Family
UniProt Gene Name
Gfra2
UniProt Synonym Gene Names
Gdnfrb; Trnr2; GDNF receptor alpha-2; GDNFR-alpha-2; GFR-alpha-2; GDNFR-beta; NRTNR-alpha; NTNR-alpha
UniProt Entry Name
GFRA2_MOUSE

NCBI Description

The protein encoded by this gene is part of the receptor complex that transduces glial cell-derived neurotrophic factor and neurturin signals by mediating autophosphorylation and activation of the RET receptor. Mice lacking this protein are viable and fertile but display growth retardation attributed to impaired salivary and pancreatic secretion and innervation deficits in the intestinal tract. In addition, knockout mice display neural defects including a failure to initiate outgrowth of dorsal ganglion root neurons, demonstrating a requirement in neuronal differentiation of these cells. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]

Uniprot Description

Function: Receptor for neurturin. Mediates the NRTN-induced autophosphorylation and activation of the RET receptor. Also able to mediate GDNF signaling through the RET tyrosine kinase receptor.

Subcellular location: Cell membrane; Lipid-anchor › GPI-anchor

By similarity.

Tissue specificity: Neurons of the superior cervical and dorsal root ganglia, and adult brain and testis. Low level in the spleen and in the adrenal gland.

Sequence similarities: Belongs to the GDNFR family.

Research Articles on GFRA2

Similar Products

Product Notes

The Mouse GFRA2 gfra2 (Catalog #AAA1752678) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1752678 ELISA Kit recognizes Mouse GFRA2. It is sometimes possible for the material contained within the vial of "GFRA2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.