Human Contactin-2 ELISA Kit | CNTN2 elisa kit

Human Contactin-2 PicoKine ELISA Kit

Cat. Num. AAA1752568

• Gene Names: CNTN2; AXT; TAX; TAX1; FAME5; TAG-1
• Reactivity: Human
• Synonyms: Contactin-2; Human Contactin-2 PicoKine ELISA Kit; Human Contactin-2; contactin 2; Axonal glycoprotein TAG-1; Axonin-1; Transient axonal glycoprotein 1; TAX-1; CNTN2; AXT; TAG1; TAX1; CNTN2 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human CNTN2
• Sequence Length: 1040

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA, citrate)
• Detection Range: 46.9pg/ml-3000pg/ml
• Sensitivity: <10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CNTN2 elisa kit

Principle of the Assay: The Picokine™ Human CNTN2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human CNTN2 with a 96-well strip plate that is pre-coated with antibody specific for CNTN2. The detection antibody is a biotinylated antibody specific for CNTN2. The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Human CNTN2 with immunogen: Expression system for standard: NSO, Immunogen sequence: S31-N1012. The kit is analytically validated with ready to use reagents. To measure Human CNTN2, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human CNTN2 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CNTN2 in the sample.!!Background/Introduction: Contactin-2 is a protein that in humans is encoded by the CNTN2 gene. It is mapped to 1q32.1. This gene encodes a member of the contactin family of proteins, part of the immunoglobulin superfamily of cell adhesion molecules. The encoded glycosylphosphatidylinositol (GPI)-anchored neuronal membrane protein plays a role in the proliferation, migration, and axon guidance of neurons of the developing cerebellum. A mutation in this gene may be associated with adult myoclonic epilepsy.

NCBI and Uniprot Product Information

• NCBI GI #: 4827022
• NCBI GeneID: 6900
• NCBI Accession #: NP_005067.1
• NCBI GenBank Nucleotide #: NP_005067.1
• UniProt Accession #: Q02246
• NCBI Official Full Name: contactin-2
• NCBI Official Synonym Full Names: contactin 2
• NCBI Official Symbol: CNTN2
• NCBI Official Synonym Symbols: AXT; TAX; TAX1; FAME5; TAG-1
• NCBI Protein Information: contactin-2
• UniProt Protein Name: Contactin-2
• Protein Family: Contactin
• UniProt Gene Name: CNTN2
• UniProt Synonym Gene Names: AXT; TAG1; TAX1; TAX-1
• UniProt Entry Name: CNTN2_HUMAN

NCBI Description

This gene encodes a member of the contactin family of proteins, part of the immunoglobulin superfamily of cell adhesion molecules. The encoded glycosylphosphatidylinositol (GPI)-anchored neuronal membrane protein plays a role in the proliferation, migration, and axon guidance of neurons of the developing cerebellum. A mutation in this gene may be associated with adult myoclonic epilepsy. [provided by RefSeq, Sep 2016]

Uniprot Description

CNTN2: May play a role in the initial growth and guidance of axons. May be involved in cell adhesion. Belongs to the immunoglobulin superfamily. Contactin family.

Protein type: Membrane protein, GPI anchor; Cell adhesion

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: voltage-gated potassium channel complex; cell surface; cell soma; integral to plasma membrane; plasma membrane; synapse; myelin sheath

Molecular Function: identical protein binding; protein self-association; carbohydrate binding; glycoprotein binding

Biological Process: axon guidance; cerebral cortex GABAergic interneuron migration; myelination in the central nervous system; positive regulation of adenosine receptor signaling pathway; axonal fasciculation; regulation of astrocyte differentiation; microtubule cytoskeleton organization and biogenesis; learning; adult walking behavior; negative regulation of neuron differentiation; receptor internalization; clustering of voltage-gated potassium channels; regulation of axon diameter; cell adhesion; regulation of neuronal synaptic plasticity

Disease: Epilepsy, Familial Adult Myoclonic, 5