Western Blot (WB)
(Biotinylated (0.1ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
Goat ILF2 / NF45 Polyclonal Antibody | anti-ILF2 antibody
Goat Anti-ILF2 / NF45, Biotinylated Antibody
Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog
Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog
Peptide ELISA: Antibody detection limit dilution 1:16000.
Western Blot (WB)
(Biotinylated (0.1ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
Western Blot (WB)
(Biotinylated (0.1ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a transcription factor required for T-cell expression of the interleukin 2 gene. It also binds RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. The encoded 45 kDa protein (NF45, ILF2) forms a complex with the 90 kDa interleukin enhancer-binding factor 3 (NF90, ILF3), and this complex has been shown to affect the redistribution of nuclear mRNA to the cytoplasm, to repair DNA breaks by nonhomologous end joining, and to negatively regulate the microRNA processing pathway. Knockdown of NF45 or NF90 protein retards cell growth, possibly by inhibition of mRNA stabilization. Alternative splicing results in multiple transcript variants. Related pseudogenes have been found on chromosomes 3 and 14. [provided by RefSeq, Dec 2014]
Uniprot Description
ILF2: Appears to function predominantly as a heterodimeric complex with ILF3. This complex may regulate transcription of the IL2 gene during T-cell activation. It can also promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA.
Protein type: RNA-binding; Nucleolus; Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 1q21.3
Cellular Component: membrane; nucleolus; nucleoplasm; nucleus; ribonucleoprotein complex
Molecular Function: DNA binding; double-stranded RNA binding; protein binding
Biological Process: positive regulation of transcription, DNA-dependent; transcription, DNA-dependent
Research Articles on ILF2
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Product Notes
The ILF2 ilf2 (Catalog #AAA423676) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat Anti-ILF2 / NF45, Biotinylated Antibody reacts with Human, Mouse, Rat, Dog Tested: Human Expected from sequence similarity: Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's ILF2 / NF45 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), PAR-CLIP. Western Blot: Approx 43kDa band observed in Human Tonsil lysates (calculated MW of 43.1kDa according to NP_004506.2). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.1-0.3ug/ml. Peptide ELISA: Antibody detection limit dilution 1:16000. Researchers should empirically determine the suitability of the ILF2 ilf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: EKAYEKPPEK KEG. It is sometimes possible for the material contained within the vial of "ILF2 / NF45, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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