Rabbit anti-Human NT5M Polyclonal Antibody | anti-NT5M antibody

NT5M Antibody (C-Term)

Cat. Num. AAA9217004

• Gene Names: NT5M; mdN; dNT2; dNT-2
• Reactivity: Human
• Applications: Western Blot
• Synonyms: NT5M; Polyclonal Antibody; NT5M Antibody (C-Term); Purified Rabbit Polyclonal Antibody (Pab); 5'(3')-deoxyribonucleotidase; mitochondrial; 5'; 3'-nucleotidase; 3.1.3.-; Deoxy-5'-nucleotidase 2; dNT-2; DNT2; anti-NT5M antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Sequence Length: 228

• Applicable Applications for anti-NT5M antibody: Western Blot (WB)
• Application Notes: WB~~1:2000
• Antigen Type: Synthesized Peptide
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-NT5M Antibody (C-Term) at 1:2000 dilutionLane 1: human liver lysateLane 2: RPMI-8226 whole cell lysateLane 3: SH-SY5Y lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size :26 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-NT5M antibody

Dephosphorylates specifically the 5' and 2'(3')- phosphates of uracil and thymine deoxyribonucleotides, and so protects mitochondrial DNA replication from excess dTTP. Has only marginal activity towards dIMP and dGMP.

Product Categories/Family for anti-NT5M antibody

Metabolism; Signal Transduction

References

Rampazzo C.,et al.Proc. Natl. Acad. Sci. U.S.A. 97:8239-8244(2000). Rinaldo-Matthis A.,et al.Nat. Struct. Biol. 9:779-787(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 9910372
• NCBI GeneID: 56953
• NCBI Accession #: NP_064586.1
• NCBI GenBank Nucleotide #: NM_020201.3
• UniProt Accession #: Q9NPB1; Q8VCE6
• Molecular Weight: 25,862 Da
• NCBI Official Full Name: 5'(3')-deoxyribonucleotidase, mitochondrial
• NCBI Official Synonym Full Names: 5',3'-nucleotidase, mitochondrial
• NCBI Official Symbol: NT5M
• NCBI Official Synonym Symbols: mdN; dNT2; dNT-2
• NCBI Protein Information: 5'(3')-deoxyribonucleotidase, mitochondrial
• UniProt Protein Name: 5'(3')-deoxyribonucleotidase, mitochondrial
• UniProt Gene Name: NT5M
• UniProt Synonym Gene Names: DNT2; 5',3'-nucleotidase, mitochondrial; dNT-2
• UniProt Entry Name: NT5M_HUMAN

NCBI Description

This gene encodes a 5' nucleotidase that localizes to the mitochondrial matrix. This enzyme dephosphorylates the 5'- and 2'(3')-phosphates of uracil and thymine deoxyribonucleotides. The gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]

Uniprot Description

NT5M: Dephosphorylates specifically the 5' and 2'(3')- phosphates of uracil and thymine deoxyribonucleotides, and so protects mitochondrial DNA replication from excess dTTP. Has only marginal activity towards dIMP and dGMP. Belongs to the 5'(3')-deoxyribonucleotidase family.

Protein type: DNA replication; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Nucleotide Metabolism - pyrimidine; EC 3.1.3.-; Nucleotide Metabolism - purine; Phosphatase (non-protein); Mitochondrial

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: mitochondrial matrix; mitochondrion

Molecular Function: 5'-nucleotidase activity; metal ion binding; nucleotidase activity; nucleotide binding

Biological Process: dephosphorylation; DNA replication; dUMP catabolic process; nucleobase, nucleoside and nucleotide metabolic process; pyrimidine base metabolic process; pyrimidine deoxyribonucleotide catabolic process; pyrimidine nucleoside catabolic process