Human GRO/MGSA CLIA Kit | GRO/MGSA clia kit

GRO/MGSA (Human) LumiAb ELISA Kit

Cat. Num. AAA9510774

• Gene Names: CXCL1; FSP; GRO1; GROa; MGSA; NAP-3; SCYB1; MGSA-a
• Reactivity: Human
• Synonyms: GRO/MGSA; GRO/MGSA (Human) LumiAb ELISA Kit; Human GRO/MGSA; Growth-regulated alpha protein; C-X-C motif chemLumine 1; GRO-alpha(1-73); Melanoma growth stimulatory activity; Neutrophil-activating protein 3; NAP-3; GROA; GRO1; SCYB1; GRO/MGSA clia kit

• Reactivity: Human
• Specificity: The LumiAb Human GM-CSF ELISA is capable of recognizing both recombinant and naturally produced Human GM-CSF proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Sequence Length: 107

• Assay Type: Quantitative Sandwich
• Sensitivity: 4 pg/ml.
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GRO/MGSA clia kit

Principle of the Assay: The LumiAb Human GM-CSF Chemiluminescent ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human GM-CSF concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human GM-CSF while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a sensitive luminescent reaction to ensue upon substrate addition. When the Peroxide Enhancer solution is added, the reaction catalyzed by peroxidase yields light that is representative of the antigen concentration. After a brief incubation, the microplate can be read with a luminometer, allowing for generation of a standard curve and subsequent determination of protein concentration!!Background/Introduction: Human GM-CSF, also known as Granulocyte-Macrophage Colony-Stimulating Factor, is a 144 amino acid cytokine protein that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Encoded by the CSF2 gene at locus 5q31.1 of chromosome 5, this gene has been localized to a cluster of related genes at the same region, which is known to be associated with the interstitial deletions in the 5q-syndrome and acute myelogenous leukemia; other genes in the clutter include those encoding IL-4, IL-5 and IL-13. After initial synthesis, the protein is proteolytically cleaved to separate the 17 residue signal sequence from the 127 GM-CSF peptide, allowing for the maturation and proper folding of the actual cytokine. The active form of the protein is often found extracellularly as a homodimer. The cytokine is also used pharmaceutically in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia.

Product Categories/Family for GRO/MGSA clia kit

Luminometer Sandwich ELISA

NCBI and Uniprot Product Information

• NCBI GI #: 4504153
• NCBI GeneID: 2919
• NCBI Accession #: NP_001502.1
• NCBI GenBank Nucleotide #: NM_001511.3
• UniProt Accession #: P09341
• NCBI Official Full Name: growth-regulated alpha protein
• NCBI Official Synonym Full Names: C-X-C motif chemokine ligand 1
• NCBI Official Symbol: CXCL1
• NCBI Official Synonym Symbols: FSP; GRO1; GROa; MGSA; NAP-3; SCYB1; MGSA-a
• NCBI Protein Information: growth-regulated alpha protein
• UniProt Protein Name: Growth-regulated alpha protein
• UniProt Gene Name: CXCL1
• UniProt Synonym Gene Names: GRO; GRO1; GROA; MGSA; SCYB1; MGSA; NAP-3
• UniProt Entry Name: GROA_HUMAN

NCBI Description

This antimicrobial gene encodes a member of the CXC subfamily of chemokines. The encoded protein is a secreted growth factor that signals through the G-protein coupled receptor, CXC receptor 2. This protein plays a role in inflammation and as a chemoattractant for neutrophils. Aberrant expression of this protein is associated with the growth and progression of certain tumors. A naturally occurring processed form of this protein has increased chemotactic activity. Alternate splicing results in coding and non-coding variants of this gene. A pseudogene of this gene is found on chromosome 4. [provided by RefSeq, Sep 2014]

Uniprot Description

CXCL1: Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO- alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q21

Cellular Component: extracellular space; extracellular region

Molecular Function: growth factor activity; chemokine activity; CXCR chemokine receptor binding; enzyme activator activity; receptor binding

Biological Process: positive regulation of catalytic activity; negative regulation of cell proliferation; cell proliferation; nervous system development; G-protein coupled receptor protein signaling pathway; immune response; response to lipopolysaccharide; positive regulation of leukocyte chemotaxis; inflammatory response; actin cytoskeleton organization and biogenesis; chemotaxis; signal transduction