Typical Testing Data/Standard Curve (for reference only)
Cathepsin G Assay Kit | CTSG assay kit
Cathepsin G Inhibitor Screening Kit (Colorimetric)
High-throughput adaptable assay to screen/study/characterize the potential inhibitors of Cathepsin G
Cathepsin G inhibitor is included in the kit
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Background: Cathepsin G (CTSG, EC 3.4.21.20) is a Serine protease found in azurophil granules of neutrophilic polymorphonuclear leukocytes. The encoded protease has specificity similar to that of chymotrypsin C, and may participate in the killing and digestion of engulfed pathogens, and in connective tissue remodeling at sites of inflammation. MyBioSource's Cathepsin G Inhibitor Screening Kit utilizes the ability of active Cathepsin G to cleave a synthetic pNA (4-Nitroaniline)-based peptide substrate to release pNA, which can be easily quantified using a colorimetric microplate reader. In the presence of a Cathepsin G inhibitor, the cleavage of this substrate is reduced/abolished resulting in decrease or total loss of the pNA absorbance. This simple and high-throughput adaptable assay kit can be used to screen/study/characterize potential inhibitors of Cathepsin G.
Summary: Detection method- Absorbance (OD 405 nm)
Applications- Screen/study/characterize potential inhibitors of Cathepsin G
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene, a member of the peptidase S1 protein family, is found in azurophil granules of neutrophilic polymorphonuclear leukocytes. The encoded protease has a specificity similar to that of chymotrypsin C, and may participate in the killing and digestion of engulfed pathogens, and in connective tissue remodeling at sites of inflammation. In addition, the encoded protein is antimicrobial, with bacteriocidal activity against S. aureus and N. gonorrhoeae. Transcript variants utilizing alternative polyadenylation signals exist for this gene. [provided by RefSeq, Sep 2014]
Uniprot Description
CTSG: Serine protease with trypsin- and chymotrypsin-like specificity. Cleaves complement C3. Has antibacterial activity against the Gram-negative bacterium P.aeruginosa, antibacterial activity is inhibited by LPS from P.aeruginosa, Z-Gly-Leu-Phe- CH2Cl and phenylmethylsulfonyl fluoride. Belongs to the peptidase S1 family.
Protein type: EC 3.4.21.20; Protease; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 14q11.2
Cellular Component: extracellular matrix; extracellular space; cell surface; extracellular region; plasma membrane; nucleus; secretory granule
Molecular Function: heparin binding; peptidase activity; serine-type endopeptidase activity
Biological Process: extracellular matrix disassembly; extracellular matrix organization and biogenesis; positive regulation of immune response; cellular protein metabolic process; immune response; protein processing; response to lipopolysaccharide; angiotensin maturation; proteolysis; defense response to fungus
Research Articles on CTSG
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Product Notes
The CTSG ctsg (Catalog #AAA841842) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. Screen/study/characterize potential inhibitors of Cathepsin G. Researchers should empirically determine the suitability of the CTSG ctsg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cathepsin G, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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