SDS-PAGE
(Figure annotation denotes ug of protein loaded and % gel used.)
Pin 1 active protein
Pin 1 protein
Applications
SDS-Page
Purity
> 95% pure
Synonyms
Pin 1; Pin 1 protein; Rotamase Pin1 protein; UBL5 protein; PPIase protein; Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 protein; Pin -1 protein; Pin -1; PPIase Pin1 protein; DOD protein; Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1; PIN1 protein; EC 5.2.1.8 protein; Pin 1 active protein
Host
Human
Purity/Purification
> 95% pure
Form/Format
Supplied as a liquid in 20mM Tris-HCl buffer, pH7.5, containing 0.1M NaCl, 5mM DTT, and 20% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence
Extracellular Ig-like domain; 22-255 amino acids: MADEEKLPPG WEKRMSRSSG RVYYFNHITN ASQWERPSGN SSSGGKNGQG EPARVRCSHL LVKHSQSRRP SSWRQEKITR TKEEALELIN GYIQKIKSGE EDFESLASQF SDCSSAKARG DLGAFSRGQM QKPFEDASFA LRTGEMSGPV FTDSGIHIIL RTE
Applicable Applications for Pin 1 active protein
SDS-PAGE
Application Notes
1. Prepare assay buffer in a suitable container and pre-chill on ice before use: The final concentrations are 35 mM Tris, pH 8.0, 1uM suc-AAFP-pNA). 2. Add recombinant Pin 1 protein at various concentrations (1ug, 2ug, 5ug) in assay buffer. 3. Mix by inversion and equilibrate to 1 DEG C and monitor the A410nm until the value is constant using a spectrophotometer. 4. Add pre-chilled chymotrypsin to 12.5 uM and mix immediately. 5. Record the increase in A410 nm for 20 minutes.
Protein Type
Recombinant
Biological Significance
Human Pin1 is a peptidyl-prolyl cis/trans isomerase (PPIase) that interacts with NIMA and essential for cell cycle regulation. Pin1 is nuclear PPIase containing a WW protein interaction domain, and is structurally and functionally related to Ess1/Ptf1, an essential protein in budding yeast. PPIase activity is necessary for Ess1/Pin1 function in yeast. Pin1 is thus an essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Substrates of Pin1 include the mitotic regulators (Cdc25 phosphatase and NIMA,PLK I, Wee, and Myt1 kinases), several transcription factors like beta-Catenin, c-Jun, and the tumor suppressor protein p53, and some specific proteins like the RNA Pol II, the cytoskeleton protein tau, and the G1/S protein Cyclin D1.
Bioactivity
Specific activity is > 162 nM/min/ug, and is defined as the amount of enzyme that cleaves 1 uM of suc-AAFP-pNA per minute at 1 degree C in Tris-Hcl pH 8.0 using chymotrypsin.
Endotoxin Levels
< 1.0 EU per ug of protein (determined by LAL method)
Expression System
E Coli
Preparation and Storage
Store at 4 degree C for short term storage (1/2 weeks). Aliquot and store at -20 degree C or - 70 degree C for long term storage. Avoid repeated freeze/thaw cycles.
SDS-PAGE
(Figure annotation denotes ug of protein loaded and % gel used.)
SDS-PAGE
(Figure annotation denotes ug of protein loaded and % gel used.)
Related Product Information for Pin 1 active protein
Purified recombinant Human Pin 1 protein
Product Categories/Family for Pin 1 active protein
Similar Products
Product Notes
The Pin 1 (Catalog #AAA836998) is an Active Protein produced from Human and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Pin 1 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. 1. Prepare assay buffer in a suitable container and pre-chill on ice before use: The final concentrations are 35 mM Tris, pH 8.0, 1uM suc-AAFP-pNA). 2. Add recombinant Pin 1 protein at various concentrations (1ug, 2ug, 5ug) in assay buffer. 3. Mix by inversion and equilibrate to 1 DEG C and monitor the A410nm until the value is constant using a spectrophotometer. 4. Add pre-chilled chymotrypsin to 12.5 uM and mix immediately. 5. Record the increase in A410 nm for 20 minutes. Researchers should empirically determine the suitability of the Pin 1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Extracellu lar Ig-like domain; 22-255 amino acids: MADEEKLPPG WEKRMSRSSG RVYYFNHITN ASQWERPSGN SSSGGKNGQG EPARVRCSHL LVKHSQSRRP SSWRQEKITR TKEEALELIN GYIQKIKSGE EDFESLASQF SDCSSAKARG DLGAFSRGQM QKPFEDASFA LRTGEMSGPV FTDSGIHIIL RTE. It is sometimes possible for the material contained within the vial of "Pin 1, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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