SDS-PAGE
(Figure annotation denotes ug of protein loaded and % gel used.)
Glutathion STransferase Mu1 Active Protein
Glutathion STransferase Mu1 protein (His tag)
Applications
SDS-Page
Purity
> 95% pure
Synonyms
Glutathion STransferase Mu1; Glutathion STransferase Mu1 protein (His tag); GST class-mu1 protein; GST 1-1 protein; GST class mu 1 protein; pmGT10 protein; Glutathione S transferase mu protein; GST class Mu protein; GSTM1 protein; GST mu protein; GST1.; Glutathion STransferase Mu1 active protein
Host
Mouse
Purity/Purification
> 95% pure
Form/Format
Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT, and 10% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence
1-218 amino acids: MGSSHHHHHH SSGLVPRGSH MPMILGYWNV RGLTHPIRML LEYTDSSYDE KRYTMGDAPD FDRSQWLNEK FKLGLDFPNL PYLIDGSHKI TQSNAILRYL ARKHHLDGET EEERIRADIV ENQVMDTRMQ LIMLCYNPDF EKQKPEFLKT IPEKMKLYSE FLGKRPWFAG DKVTYVDFLA YDILDQYRMF EPKCLDAFPN LRDFLARFEG LKKISAYMKS SRYIATPIFS KMAHWSNK
Applicable Applications for Glutathion STransferase Mu1 active protein
SDS-PAGE
Application Notes
1. Prepare a 1ml reaction mix into a suitable container: The final concentrations are 97 mM potassium phosphate, 0.97 mM EDTA, 2.5 mM glutathione, reduced, 1.0 mM 1-chloro-2,4-dinitrobenzene (CDNB), 3.2% (v/v) ethanol. 2. Equilibrate to 25 DEG C and monitor at A340nm until the value is constant using a spectrophotometer. 3. Add 50ul of recombinant mouse GSTM1 protein with various concentrations (1ug, 2ug, 5ug) in 950ul reaction buffer. 4. Mix by inversion and record the increase at A340nm for 5 minutes.
Protein Type
Recombinant
Biological Significance
GSTM1 is a glutathione S-transferase that belongs to the mu class. This enzyme acts by catalyzing the reaction of glutathione with an acceptor molecule to form an S-substituted glutathione (S=sulfur). The reactions utilizing glutathione contribute the transformation of a wide variety of electrophiles, including reactive products of lipid, protein, carcinogens, therapeutic drugs, environmental toxins, and products of oxidative stress. Recombinant GSTM1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Bioactivity
Specific activity is < 11 units/mg, and is defined as the amount of enzyme that conjugate 1.0 uM of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25 degree C.
Expression System
E Coli
Tag/Conjugate
His tag
Preparation and Storage
Store at 4 degree C for short term storage (1/2 weeks). Aliquot and store at -20 degree C or - 70 degree C for long term storage. Avoid repeated freeze/thaw cycles.
SDS-PAGE
(Figure annotation denotes ug of protein loaded and % gel used.)
SDS-PAGE
(Figure annotation denotes ug of protein loaded and % gel used.)
Related Product Information for Glutathion STransferase Mu1 active protein
Purified recombinant Mouse Glutathion STransferase Mu1 protein
Product Categories/Family for Glutathion STransferase Mu1 active protein
Similar Products
Product Notes
The Glutathion STransferase Mu1 (Catalog #AAA536754) is an Active Protein produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Glutathion STransferase Mu1 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. 1. Prepare a 1ml reaction mix into a suitable container: The final concentrations are 97 mM potassium phosphate, 0.97 mM EDTA, 2.5 mM glutathione, reduced, 1.0 mM 1-chloro-2,4-dinitrobenzene (CDNB), 3.2% (v/v) ethanol. 2. Equilibrate to 25 DEG C and monitor at A340nm until the value is constant using a spectrophotometer. 3. Add 50ul of recombinant mouse GSTM1 protein with various concentrations (1ug, 2ug, 5ug) in 950ul reaction buffer. 4. Mix by inversion and record the increase at A340nm for 5 minutes. Researchers should empirically determine the suitability of the Glutathion STransferase Mu1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: 1-218 amino acids: MGSSHHHHHH SSGLVPRGSH MPMILGYWNV RGLTHPIRML LEYTDSSYDE KRYTMGDAPD FDRSQWLNEK FKLGLDFPNL PYLIDGSHKI TQSNAILRYL ARKHHLDGET EEERIRADIV ENQVMDTRMQ LIMLCYNPDF EKQKPEFLKT IPEKMKLYSE FLGKRPWFAG DKVTYVDFLA YDILDQYRMF EPKCLDAFPN LRDFLARFEG LKKISAYMKS SRYIATPIFS KMAHWSNK. It is sometimes possible for the material contained within the vial of "Glutathion STransferase Mu1, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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