SDS-PAGE
(Figure annotation denotes ug of protein loaded and % gel used.)
PPIF active protein
PPIF protein (His tag)
Gene Names
PPIF; CYP3; CypD; CyP-M; Cyp-D
Applications
SDS-Page
Purity
> 95% pure
Synonyms
PPIF; PPIF protein (His tag); Peptidylprolyl isomerase F Cyclophilin 3 protein; Peptidylprolyl isomerase F (cyclophilin F) protein; Rotamase.; Peptidyl prolyl cis trans isomerase protein; Cyclophilin F protein; Cyclophilin D protein; CYP3 protein; Peptidylprolyl isomerase F protein; PPIase protein; mitochondral protein; hCyP3 protein; Cyp D protein; PPIF protein; CypD protein; Cyp-D protein; PPIF active protein
Host
Human
Purity/Purification
> 95% pure
Form/Format
Supplied as a liquid in 20mM Tris, pH 7.5, containing 1mM DTT, and 10% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence
30-207 amino acids: MGSSHHHHHH SSGLVPRGSH CSKGSGDPSS SSSSGNPLVY LDVDANGKPL GRVVLELKAD VVPKTAENFR ALCTGEKGFG YKGSTFHRVI PSFMCQAGDF TNHNGTGGKS IYGSRFPDEN FTLKHVGPGV LSMANAGPNT NGSQFFICTI KTDWLDGKHV VFGHVKEGMD VVKKIESFGS KSGRTSKKIV ITDCGQLS
Sequence Length
207
Applicable Applications for PPIF active protein
SDS-PAGE
Application Notes
1. Prepare assay buffer in a suitable container and pre-chill on ice before use: The final concentrations are 35 mM Tris, pH 8.0, 1uM suc-AAFP-pNA). 2. Add recombinant Cyclophilin F protein with various concentrations (1ug, 2ug, 5ug) in assay buffer. 3. Mix by inversion and equilibrate to 1 DEG C and monitor the A410nm until the value is constant using a spectrophotometer. 4. Add pre-chilled chymotrypsin to 12.5 uM and mix immediately. 5. Record the increase in A410 nm for 20 minutes
Protein Type
Recombinant
Biological Significance
Cyclophilin F (also known as Peptidylprolyl isomerase F, PPIF) is a member of peptidyl-prolyl cis-trans isomerase family. They are highly-conserved cytoplasmic enzymes that accelerate protein folding. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death. Recombinant PPIF protein was expressed in E Coli and purified by using conventional chromatography techniques.
Bioactivity
Specific activity is > 120 nM/min/ug, and is defined as the amount of enzyme that cleaves 1uM of suc-AAFP-pNA per minute at 1 degree C in Tris-Hcl pH 8.0 using chymotrypsin.
Endotoxin Levels
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Expression System
E Coli
Tag/Conjugate
His tag
Preparation and Storage
Store at 4 degree C for short term storage (1/2 weeks). Aliquot and store at -20 degree C or - 70 degree C for long term storage. Avoid repeated freeze/thaw cycles.
SDS-PAGE
(Figure annotation denotes ug of protein loaded and % gel used.)
SDS-PAGE
(Figure annotation denotes ug of protein loaded and % gel used.)
Related Product Information for PPIF active protein
Purified recombinant Human PPIF protein
Product Categories/Family for PPIF active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21 kDa (198aa), confirmed by MALDI-TOF.
NCBI Official Full Name
peptidyl-prolyl cis-trans isomerase F, mitochondrial
NCBI Official Synonym Full Names
peptidylprolyl isomerase F
NCBI Official Symbol
PPIF
NCBI Official Synonym Symbols
CYP3; CypD; CyP-M; Cyp-D
NCBI Protein Information
peptidyl-prolyl cis-trans isomerase F, mitochondrial
UniProt Protein Name
Peptidyl-prolyl cis-trans isomerase F, mitochondrial
Protein Family
UniProt Gene Name
PPIF
UniProt Synonym Gene Names
CYP3; PPIase F; CyP-D; CypD; CyP-M
UniProt Entry Name
PPIF_HUMAN
NCBI Description
The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death. [provided by RefSeq, Jul 2008]
Uniprot Description
PPIF: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Belongs to the cyclophilin-type PPIase family.
Protein type: EC 5.2.1.8; Isomerase; Mitochondrial
Chromosomal Location of Human Ortholog: 10q22.3
Cellular Component: membrane; mitochondrial matrix; mitochondrial proton-transporting ATP synthase complex
Molecular Function: protein binding; peptidyl-prolyl cis-trans isomerase activity; cyclosporin A binding
Biological Process: apoptotic mitochondrial changes; protein peptidyl-prolyl isomerization; protein folding; regulation of mitochondrial membrane permeability; negative regulation of ATPase activity; negative regulation of apoptosis
Research Articles on PPIF
Similar Products
Product Notes
The PPIF ppif (Catalog #AAA836606) is an Active Protein produced from Human and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's PPIF can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. 1. Prepare assay buffer in a suitable container and pre-chill on ice before use: The final concentrations are 35 mM Tris, pH 8.0, 1uM suc-AAFP-pNA). 2. Add recombinant Cyclophilin F protein with various concentrations (1ug, 2ug, 5ug) in assay buffer. 3. Mix by inversion and equilibrate to 1 DEG C and monitor the A410nm until the value is constant using a spectrophotometer. 4. Add pre-chilled chymotrypsin to 12.5 uM and mix immediately. 5. Record the increase in A410 nm for 20 minutes. Researchers should empirically determine the suitability of the PPIF ppif for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: 30-207 amino acids: MGSSHHHHHH SSGLVPRGSH CSKGSGDPSS SSSSGNPLVY LDVDANGKPL GRVVLELKAD VVPKTAENFR ALCTGEKGFG YKGSTFHRVI PSFMCQAGDF TNHNGTGGKS IYGSRFPDEN FTLKHVGPGV LSMANAGPNT NGSQFFICTI KTDWLDGKHV VFGHVKEGMD VVKKIESFGS KSGRTSKKIV ITDCGQLS. It is sometimes possible for the material contained within the vial of "PPIF, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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