FKBP6 active protein

FKBP6, 1-327aa, Human, His tag, E Coli (Bioactivity Validated)

Cat. Num. AAA203571

• Gene Names: FKBP6; FKBP36
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: FKBP6; 1-327aa; Human; His tag; E Coli (Bioactivity Validated); FK506 binding protein 6 36kDa; FKBP36; PPIase FKBP6; FK506 binding protein 6; 36kDa 36 kDa FK506 binding protein; EC 5.2.1.8; FKBP 36; Immunophilin FKBP36; Peptidyl prolyl cis trans isomerase; PPIase; Rotamase.; FKBP6 active protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 40% glycerol, 1mM EDTA, 50mM NaCl
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGGSALNQGV LEGDDAPGQS LYERLSQRML DISGDRGVLK DVIREGAGDL VAPDASVLVK YSGYLEHMDR PFDSNYFRKT PRLMKLGEDI TLWGMELGLL SMRRGELARF LFKPNYAYGT LGCPPLIPPN TTVLFEIELL DFLDCAESDK FCALSAEQQD QFPLQKVLKV AATEREFGNY LFRQNRFYDA KVRYKRALLL LRRRSAPPEE QHLVEAAKLP VLLNLSFTYL KLDRPTIALC YGEQALIIDQ KNAKALFRCG QACLLLTEYQ KARDFLVRAQ KEQPFNHDIN NELKKLASCY RDYVDKEKEM WHRMFAPCGD GSTAGES
• Sequence Length: 327

• Applicable Applications for FKBP6 active protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Biological Activity: Specific activity is > 190 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25C in Tris-Hcl pH8.0 using chymotrypsin.
• Assay: 1. Prepare 170ul assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin.; 2. Add 10ul of recombinant FKBP6 protein with 1ug in assay buffer.; 3. Mix by inversion and equilibrate to 1C and monitor the A405nm until the value is constant using a spectrophotometer.; 4. Add 20ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM); 5. Record the increase in A405 nm for 30 minutes at 25C.
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for FKBP6 active protein

FK506 binding protein 6, also known as FKBP6, is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. ubiquitously expressed in all tissues, FKBP6 is present at highest levels in testis, liver, kidney, skeletal muscle and heart. Deletion of FKBP6 may contribute to hypercalcemia and growth delay in Williams-Beuren syndrome. Recombinant human FKBP6, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for FKBP6 active protein

Immunology

References

Crackower MA., et al. (2003) Science. 300 (5623):1231-5.; Metcalfe K., et al. (2005) Clin Dysmorphpl. 14 (2): 61-5.;

NCBI and Uniprot Product Information

• NCBI GI #: 146229346
• NCBI GeneID: 8468
• NCBI Accession #: NP_003593
• NCBI GenBank Nucleotide #: NM_003602.4
• UniProt Accession #: O75344; Q7Z4T4; Q9UDS0; B4DXT7; G3V0I2
• Molecular Weight: 39.3 kDa (347aa), confirmed by MALDI-TOF
• NCBI Official Full Name: inactive peptidyl-prolyl cis-trans isomerase FKBP6 isoform a
• NCBI Official Synonym Full Names: FK506 binding protein 6
• NCBI Official Symbol: FKBP6
• NCBI Official Synonym Symbols: FKBP36
• NCBI Protein Information: inactive peptidyl-prolyl cis-trans isomerase FKBP6
• UniProt Protein Name: Inactive peptidyl-prolyl cis-trans isomerase FKBP6
• Protein Family: FK506-binding protein
• UniProt Gene Name: FKBP6
• UniProt Synonym Gene Names: FKBP36; Inactive PPIase FKBP6; 36 kDa FKBP; FKBP-36; FKBP-6

NCBI Description

The protein encoded by this gene is a cis-trans peptidyl-prolyl isomerase that may function in immunoregulation and basic cellular processes involving protein folding and trafficking. This gene is located in a chromosomal region that is deleted in Williams-Beuren syndrome. Defects in this gene may cause male infertility. There are multiple pseudogenes for this gene located nearby on chromosome 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

Co-chaperone required during spermatogenesis to repress transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Acts as a co-chaperone via its interaction with HSP90 and is required for the piRNA amplification process, the secondary piRNA biogenesis. May be required together with HSP90 in removal of 16 nucleotide ping-pong by-products from Piwi complexes, possibly facilitating turnover of Piwi complexes ().