PPIL3 active protein

PPIL3, 1-161aa, Human, His tag, E Coli (Bioactivity Validated)

Cat. Num. AAA204620

• Gene Names: PPIL3; CYPJ
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: PPIL3; 1-161aa; Human; His tag; E Coli (Bioactivity Validated); Peptidyl-prolyl cis-trans isomerase-like 3 isoform PPIL3b; CYPJ; PPIL3 active protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT
• Concentration: 1.0 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MSVTLHTDVG DIKIEVFCER TPKTCENFLA LCASNYYNGC IFHRNIKGFM VQTGDPTGTG RGGNSIWGKK FEDEYSEYLK HNVRGVVSMA NNGPNTNGSQ FFITYGKQPH LDMKYTVFGK VIDGLETLDE LEKLPVNEKT YRPLNDVHIK DITIHANPFA Q
• Sequence Length: 161

• Applicable Applications for PPIL3 active protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Biological Activity: Specific activity is > 280 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25C in Tris-Hcl pH8.0 using chymotrypsin.
• Assay: 1. Prepare 170ul assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin.; 2. Add 10ul of recombinant PPIL3 protein with 1ug in assay buffer.; 3. Mix by inversion and equilibrate to 1C and monitor the A405nm until the value is constant using a spectrophotometer.; 4. Add 20ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM); 5. Record the increase in A405 nm for 30 minutes at 25C.
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for PPIL3 active protein

PPIL3, also known as peptidyl-prolyl cis-trans isomerase-like 3, is a member of the cyclophilin family that catalyzes the cis-trans isomerization of peptidylprolyl imide bonds in oligopeptides. It has been proposed to act either as catalysts or as molecular chaperones in protein-folding events. Recombinant human PPIL3 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography.

Product Categories/Family for PPIL3 active protein

Heat Shock Proteins

References

Zhou Z. et al. (2001) Cytogenet Cell Genet. 92: 231-236; Jurica MS. et al. (2002) RNA. 8:426-439.

NCBI and Uniprot Product Information

• NCBI GI #: 19557636
• NCBI GeneID: 53938
• NCBI Accession #: NP_570981
• NCBI GenBank Nucleotide #: NM_130906.2
• UniProt Accession #: Q9H2H8; Q86WF9; Q96IA9; Q9BXZ1
• Molecular Weight: 20.3 kDa (181aa) confirmed by MALDI-TOF
• NCBI Official Full Name: peptidyl-prolyl cis-trans isomerase-like 3 isoform PPIL3b
• NCBI Official Synonym Full Names: peptidylprolyl isomerase like 3
• NCBI Official Symbol: PPIL3
• NCBI Official Synonym Symbols: CYPJ
• NCBI Protein Information: peptidyl-prolyl cis-trans isomerase-like 3
• UniProt Protein Name: Peptidyl-prolyl cis-trans isomerase-like 3
• Protein Family: Peptidyl-prolyl cis-trans isomerase
• UniProt Gene Name: PPIL3
• UniProt Synonym Gene Names: PPIase; CyPJ

NCBI Description

This gene encodes a member of the cyclophilin family. Cyclophilins catalyze the cis-trans isomerization of peptidylprolyl imide bonds in oligopeptides. They have been proposed to act either as catalysts or as molecular chaperones in protein-folding events. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2008]

Uniprot Description

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. May be involved in pre-mRNA splicing.