PPIL4 active protein

PPIL4, 1-492aa, Human, His tag, E Coli (Bioactivity Validated)

Cat. Num. AAA203964

• Gene Names: PPIL4; HDCME13P
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: PPIL4; 1-492aa; Human; His tag; E Coli (Bioactivity Validated); Peptidyl-prolyl cis-trans isomerase-like 4; HDCME13P; Rotamase PPIL4; Cyclophilin-like protein PPIL4; PPIL4 active protein

• Host: E Coli
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl.
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MAVLLETTLG DVVIDLYTEE RPRACLNFLK LCKIKYYNYC LIHNVQRDFI IQTGDPTGTG RGGESIFGQL YGDQASFFEA EKVPRIKHKK KGTVSMVNNG SDQHGSQFLI TTGENLDYLD GVHTVFGEVT EGMDIIKKIN ETFVDKDFVP YQDIRINHTV ILDDPFDDPP DLLIPDRSPE PTREQLDSGR IGADEEIDDF KGRSAEEVEE IKAEKEAKTQ AILLEMVGDL PDADIKPPEN VLFVCKLNPV TTDEDLEIIF SRFGPIRSCE VIRDWKTGES LCYAFIEFEK EEDCEKAFFK MDNVLIDDRR IHVDFSQSVA KVKWKGKGGK YTKSDFKEYE KEQDKPPNLV LKDKVKPKQD TKYDLILDEQ AEDSKSSHSH TSKKHKKKTH HCSEEKEDED YMPIKNTNQD IYREMGFGHY EEEESCWEKQ KSEKRDRTQN RSRSRSRERD GHYSNSHKSK YQTDLYERER SKKRDRSRSP KKSKDKEKSK YR
• Sequence Length: 492

• Applicable Applications for PPIL4 active protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Biological Activity: Specific activity is > 190 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25C in Tris-Hcl pH8.0 using chymotrypsin
• Assay: 1. Prepare 170ul assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin.; 2. Add 10ul of recombinant PPIL4 protein with 1ug in assay buffer.; 3. Mix by inversion and equilibrate to 1C and monitor the A405nm until the value is constant using a spectrophotometer.; 4. Add 20ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM); 5. Record the increase in A405 nm for 30 minutes at 25C.
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for PPIL4 active protein

PPIL4, also known as HDCME13P, is evolutionarily conserved member of the cyclophilin-type PPIase family of proteins. ubiquitously expressed with predominant expression in kidney, PPIL4 localizes to the nucleus and contains one PPIase cyclophilin-type domain, a lysine-rich domain, a pair of bipartite nuclear targeting sequences and one RRM (RNA recognition motif) domain. The presence of the RRM domain along with nuclear targeting sequences suggests that PPIL4 may be involved in transcriptional regulation. Recombinant human PPIL4 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for PPIL4 active protein

Heat Shock Proteins

References

Nebert DW., et al. (2004) Hum Genomics. 1 (5):381-8. ; Valle C., et al. (2005) Parasitol Res. 96 (4):199-205.;

NCBI and Uniprot Product Information

• NCBI GI #: 20911035
• NCBI GeneID: 85313
• NCBI Accession #: NP_624311
• NCBI GenBank Nucleotide #: NM_139126.3
• UniProt Accession #: Q8WUA2; Q7Z3Q5; B2RD34
• Molecular Weight: 59.4 kDa (512aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
• NCBI Official Full Name: peptidyl-prolyl cis-trans isomerase-like 4
• NCBI Official Synonym Full Names: peptidylprolyl isomerase like 4
• NCBI Official Symbol: PPIL4
• NCBI Official Synonym Symbols: HDCME13P
• NCBI Protein Information: peptidyl-prolyl cis-trans isomerase-like 4
• UniProt Protein Name: Peptidyl-prolyl cis-trans isomerase-like 4
• Protein Family: Peptidyl-prolyl cis-trans isomerase
• UniProt Gene Name: PPIL4
• UniProt Synonym Gene Names: PPIase

NCBI Description

This gene is a member of the cyclophilin family of peptidylprolyl isomerases. The cyclophilins are a highly conserved family, members of which play an important role in protein folding, immunosuppression by cyclosporin A, and infection of HIV-1 virions. [provided by RefSeq, Jul 2008]

Uniprot Description

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides ().