PPIL2 active protein

PPIL2, 1-527aa, Human, His tag, E Coli (Bioactivity Validated)

Cat. Num. AAA203468

• Gene Names: PPIL2; CYC4; CYP60; UBOX7; Cyp-60; hCyP-60
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: PPIL2; 1-527aa; Human; His tag; E Coli (Bioactivity Validated); Peptidyl-prolyl cis-trans isomerase-like 2; CYC4; Cyp-60; CYP60; hCyP-60; GC33174; Peptidyl-prolyl cis-trans isomerase-like 2 Cyclophilin 60; Cyclophilin like protein Cyp 60; Peptidyl prolyl cis trans isomerase like 2; PPIase; Rotamase PPIL2.; PPIL2 active protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGKRQHQKDK MYITCAEYTH FYGGKKPDLP QTNFRRLPFD HCSLSLQPFV YPVCTPDGIV FDLLNIVPWL KKYGTNPSNG EKLDGRSLIK LNFSKNSEGK YHCPVLFTVF TNNTHIVAVR TTGNVYAYEA VEQLNIKAKN FRDLLTDEPF SRQDIITLQD PTNLDKFNVS NFYHVKNNMK IIDPDEEKAK QDPSYYLKNT NAETRETLQE LYKEFKGDEI LAATMKAPEK KKVDKLNAAH YSTGKVSASF TSTAMVPETT HEAAAIDEDV LRYQFVKKKG YVRLHTNKGD LNLELHCDLT PKTCENFIRL CKKHYYDGTI FHRSIRNFVI QGGDPTGTGT GGESYWGKPF KDEFRPNLSH TGRGILSMAN SGPNSNRSQF FITFRSCAYL DKKHTIFGRV VGGFDVLTAM ENVESDPKTD RPKEEIRIDA TTVFVDPYEE ADAQIAQERK TQLKVAPETK VKSSQPQAGS QGPQTFRQGV GKYINPAATE QQRKSPQPVP LSPCPRRSPV GVLGTSAPGS SRLPDDH
• Sequence Length: 527

• Applicable Applications for PPIL2 active protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Biological Activity: Specific activity is > 290 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25C in Tris-Hcl pH8.0 using chymotrypsin..
• Assay: 1. Prepare 170ul assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin.; 2. Add 10ul of recombinant PPIL2 protein with 1ug in assay buffer.; 3. Mix by inversion and equilibrate to 1C and monitor the A405nm until the value is constant using a spectrophotometer.; 4. Add 20ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM); 5. Record the increase in A405 nm for 30 minutes at 25C.
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for PPIL2 active protein

PPIL2, which belongs to the cyclophilin family of peptidylprolyl isomerases, is an enzyme that plays an important role in protein folding, immunosuppression by cyclosporin A, and infection of HIV-1 virions. This protein interacts with the proteinase inhibitor eglin c and is localized in the nucleus. Recombinant PPIL2 protein was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for PPIL2 active protein

Heat Shock Proteins

References

Wang BB, et al. (1996) Biochem J. 314:313-9.; Carson R, et al. (2009) Neuromolecular Med. 11 (4):337-44.;

NCBI and Uniprot Product Information

• NCBI GI #: 22547215
• NCBI GeneID: 23759
• NCBI Accession #: NP_680481
• NCBI GenBank Nucleotide #: NM_148176.2
• UniProt Accession #: Q13356; Q13357; Q8TAH2; Q9BWR8
• Molecular Weight: 61.6 kDa (547aa)
• NCBI Official Full Name: peptidyl-prolyl cis-trans isomerase-like 2 isoform b
• NCBI Official Synonym Full Names: peptidylprolyl isomerase like 2
• NCBI Official Symbol: PPIL2
• NCBI Official Synonym Symbols: CYC4; CYP60; UBOX7; Cyp-60; hCyP-60
• NCBI Protein Information: peptidyl-prolyl cis-trans isomerase-like 2
• UniProt Protein Name: Peptidyl-prolyl cis-trans isomerase-like 2
• Protein Family: Peptidyl-prolyl cis-trans isomerase
• UniProt Gene Name: PPIL2
• UniProt Synonym Gene Names: hCyP-60

NCBI Description

This gene is a member of the cyclophilin family of peptidylprolyl isomerases. The cyclophilins are a highly conserved ubiquitous family, members of which play an important role in protein folding, immunosuppression by cyclosporin A, and infection of HIV-1 virions. This protein interacts with the proteinase inhibitor eglin c and is localized in the nucleus. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2015]

Uniprot Description

May catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides thereby assisting the folding of proteins (). May also function as a chaperone, playing a role in transport to the cell membrane of BSG for instance (PubMed:15946952). May also have a protein ubiquitin ligase activity acting as an E3 ubiquitin protein ligase or as an ubiquitin-ubiquitin ligase promoting elongation of ubiquitin chains on substrates. By mediating 'Lys-48'-linked polyubiquitination of proteins could target them for proteasomal degradation (PubMed:11435423).