Carbonic Anhydrase X Active Protein | CA10 active protein

Carbonic Anhydrase X Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122976

• Gene Names: Car10; Ca10; BB085816; 2700029L05Rik
• Purity: >97% as determined by SDS-PAGE
• Synonyms: Carbonic Anhydrase X; Carbonic Anhydrase X Protein; Mouse; Recombinant (His Tag); Mouse Carbonic Anhydrase X/Car10 Protein (His Tag); 2700029L05Rik Protein; BB085816 Protein; Ca10 Protein; Car10 Protein; RP23-448I9.1 Protein; carbonic anhydrase X; CA10 active protein

• Host: HEK293 Cells
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 25mM Tris, 150mM NaCl, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Asn300

• Species: Mouse
• Activity: Measured by its esterase activity. The specific activity is >10pmoles/min/ug.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gln 23
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse Car10 (P61215)(Met 1-Asn 300) was expressed, fused with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CA10 active protein

Background: Carbonic anhydrase X, also called carbonic anhydrase - related protein X (CARPX) and CA1, belongs to the CA family of zinc metalloenzymes which catalyze the reversible hydration of carbon dioxide in various biological processes such as respiration, renal tubular acidification and bone resorption. The secreted protein CARPX without CA activity (hydration of CO2) is identified as an acatalytic member of the alpha-carbonic anhydrase subgroup. CARP X expression is detected in the adult total brain and almost all parts of the central nervous system, but not in the fetal brain. Accordingly, CARP X is suggested to play a role in the development of central nervous system, especially the brain. The same CARP X protein are encoded by multiple transcript variants of this gene.

References

Okamoto, N. et al., 2001, Biochim. Biophys. Acta 1518:311-316. Taniuchi, K. et al., Neuroscience 112: 93-99. Supuran, C.T. et al., 2003, Med. Res. Rev. 23: 146-189.

NCBI and Uniprot Product Information

• NCBI GI #: 58037303
• NCBI GeneID: 72605
• NCBI Accession #: NP_082572.1
• NCBI GenBank Nucleotide #: NM_028296.3
• UniProt Accession #: P61215
• Molecular Weight: 37,563 Da
• NCBI Official Full Name: carbonic anhydrase-related protein 10
• NCBI Official Synonym Full Names: carbonic anhydrase 10
• NCBI Official Symbol: Car10
• NCBI Official Synonym Symbols: Ca10; BB085816; 2700029L05Rik
• NCBI Protein Information: carbonic anhydrase-related protein 10
• UniProt Protein Name: Carbonic anhydrase-related protein 10
• Protein Family: Carbonic anhydrase-related protein
• UniProt Gene Name: Ca10
• UniProt Synonym Gene Names: Car10; CA-RP X; CARP X

Uniprot Description

CA10: Does not have a catalytic activity. Belongs to the alpha-carbonic anhydrase family.

Chromosomal Location of Human Ortholog: 11|11 D