Testing Data
Carbonic Anhydrase 10/CA10 Active Protein | CA10 active protein
Human CellExp Carbonic Anhydrase 10 / CA10, human recombinant
Gene Names
CA10; CARPX; CA-RPX; HUCEP-15
Applications
SDS-Page
Purity
>=95%
Synonyms
Carbonic Anhydrase 10/CA10; Human CellExp Carbonic Anhydrase 10 / CA10; human recombinant; CA10; Carbonic Anhydrase X; CARPX; hucep-15; CA10 active protein
Host
HEK293 cells
Purity/Purification
>=95%
Form/Format
Lyophilized from 0.22 mum filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5. Normally Mannitol or Trehalose is added as protectants before lyophilization.
Appearance: Lyophilized
Appearance: Lyophilized
Sequence Length
328
Applicable Applications for CA10 active protein
SDS-PAGE
Solubility/Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 ug/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 month. For extended storage, it is recommended to store at -20 degree C.
Endotoxin Level
<1 EU/mug by LAL method
Biological Activity
Measured by its esterase activity. The specific activity, as measured with 1 mM 4-Nitrophenyl acetate (4-NPA) as substrate and 5 mug rh CA10 / CARPX enzyme at 100 ng/ul in 100 muL of 12.5 mM Tris, 75 mM NaCl, pH 7.5. The specific activity is >1 pmol/min/ug.
Results
Measured by its esterase activity. The specific activity, as measured with 1 mM 4-Nitrophenyl acetate (4-NPA) as substrate and 5 mug rh CA10 / CARPX enzyme at 100 ng/ul in 100 muL of 12.5 mM Tris, 75 mM NaCl, pH 7.5. The specific activity is >1 pmol/min/ug.
Handling
Centrifuge the vial prior to opening.
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Testing Data
Testing Data
Related Product Information for CA10 active protein
Background: Carbonic anhydrase-related protein 10 (CA10) is also known as Carbonic anhydrase-related protein X (CARP X or CARPX), Cerebral protein 15 (hucep-15), which belongs to the alpha-carbonic anhydrase family of zinc metalloenzymes, which catalyze the reversible hydration of carbon dioxide in various biological processes. CA10 / CARPX is an acatalytic member of the alpha-carbonic anhydrase subgroup, and it is thought to play a role in the central nervous system, especially in brain development.
Product Categories/Family for CA10 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
This protein is fused with polyhistidine tag at the C-terminus, has a calculated MW of 32.6 kDa. The predicted N-terminus is Gln 22. DTT-reduced Protein migrates as 37 kDa due to glycosylation.
NCBI Official Full Name
carbonic anhydrase-related protein 10
NCBI Official Synonym Full Names
carbonic anhydrase X
NCBI Official Symbol
CA10
NCBI Official Synonym Symbols
CARPX; CA-RPX; HUCEP-15
NCBI Protein Information
carbonic anhydrase-related protein 10
UniProt Protein Name
Carbonic anhydrase-related protein 10
Protein Family
UniProt Gene Name
CA10
UniProt Synonym Gene Names
CA-RP X; CARP X
UniProt Entry Name
CAH10_HUMAN
NCBI Description
This gene encodes a protein that belongs to the carbonic anhydrase family of zinc metalloenzymes, which catalyze the reversible hydration of carbon dioxide in various biological processes. The protein encoded by this gene is an acatalytic member of the alpha-carbonic anhydrase subgroup, and it is thought to play a role in the central nervous system, especially in brain development. Multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
CA10: Does not have a catalytic activity. Belongs to the alpha-carbonic anhydrase family.
Chromosomal Location of Human Ortholog: 17q21.33
Biological Process: brain development
Research Articles on CA10
Similar Products
Product Notes
The CA10 ca10 (Catalog #AAA845192) is an Active Protein produced from HEK293 cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Carbonic Anhydrase 10/CA10 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the CA10 ca10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Carbonic Anhydrase 10/CA10, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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