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Carbonic Anhydrase VII Active Protein | CA7 active protein
Carbonic Anhydrase VII Protein, Human, Recombinant (His Tag)
Purity
>90% as determined by SDS-PAGE
Synonyms
Carbonic Anhydrase VII; Carbonic Anhydrase VII Protein; Human; Recombinant (His Tag); Human Carbonic Anhydrase VII/CA7 Protein (His Tag); CAVII Protein; carbonic anhydrase VII; CA7 active protein
Host
E Coli
Purity/Purification
>90% as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Sequence
Met1-Ala264
Species
Human
Activity
Measured by its esterase activity. The activity is >20pmoles/min/ug.
Predicted N Terminal
Met
Tag
C-His
Protein Construction
A DNA sequence encoding the human CA7 (P43166)(Met 1-Ala 264) was fused with a polyhistide tag at the C-terminus.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
SDS-Page
SDS-Page
Related Product Information for CA7 active protein
Background: Carbonic anhydrase 7, also known as carbonate dehydratase VII, carbonic anhydrase VII, CA-VII and CA7, is a cytoplasm protein which belongs to thealpha-carbonic anhydrase family. Carbonic anhydrases are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. Carbonic anhydrases show extensive diversity in tissue distribution and in their subcellular localization. CA7/CA-VII is predominantly expressed in the salivary glands. Alternative splicing in the coding region results in multiple transcript variants encoding different isoforms.
References
Montgomery J.C., et al., 1991, Genomics 11:835-848. Temperini C., et al., 2006, Chemistry 12:7057-7066. Temperini C., et al., 2007, Bioorg. Med. Chem. Lett. 17:628-635. Maresca A., et al., 2009, J. Am. Chem. Soc. 131:3057-3062.
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
The recombinant human CA7 consisting of 274 amino acids and has a calculated molecular mass of 31kDa. It migrates as an approximately 33kDa band in SDS-PAGE under reducing conditions.
UniProt Protein Name
Carbonic anhydrase 7
Protein Family
UniProt Gene Name
CA7
UniProt Entry Name
CAH7_HUMAN
Similar Products
Product Notes
The CA7 ca7 (Catalog #AAA8121102) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Met1-Ala26 4. It is sometimes possible for the material contained within the vial of "Carbonic Anhydrase VII, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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