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Carbonic anhydrase II Active Protein | CA2 active protein
Carbonic anhydrase II Protein, Mouse, Recombinant (His Tag)
Gene Names
Car2; Ca2; CAII; Car-2; Ltw-5; Lvtw-5; AI131712
Purity
>96% as determined by SDS-PAGE
Synonyms
Carbonic anhydrase II; Carbonic anhydrase II Protein; Mouse; Recombinant (His Tag); Mouse Carbonic Anhydrase II/Car2 Protein (His Tag); AI131712 Protein; Ca2 Protein; CAII Protein; Car-2 Protein; Ltw-5 Protein; Lvtw-5 Protein; carbonic anhydrase II; CA2 active protein
Host
E Coli
Purity/Purification
>96% as determined by SDS-PAGE
Form/Format
Lyophilized from sterile 50mM Tris, 150mM NaCl, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Sequence
Met1-Lys260
Species
Mouse
Activity
Measured by its esterase activity. The specific activity is >100pmoles/min/ug.
Predicted N Terminal
Ser 2
Tag
C-His
Protein Construction
A DNA sequence encoding the mouse CA2 (AAH55291.1)(Met 1-Lys 260) was expressed, with a C-terminal polyhistidine tag.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
SDS-Page
SDS-Page
Related Product Information for CA2 active protein
Background: The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase II is one of fourteen forms of human alpha carbonic anhydrases. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Renal carbonic anhydrase allows the reabsorption of sodium ions in the proximal tubule. Carbonic anhydrase II has been shown to interact with Band 3 and Sodium-hydrogen antiporter 1.
References
Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27.Lindskog S. (1997) Structure and mechanism of carbonic anhydrase. Pharmacology & Therapeutics. 74(1): 1-20>Lilias A, et al. (1972) Crystal Structure of Human Carbonic Anhydrase C. Nature new biology. 235: 131-7.Li XJ, et al. (2002) Carbonic Anhydrase II Binds to and Enhances Activity of the Na+/H+ Exchanger. The Journal of Biological Chemistry. 277: 36085-91.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
29,033 Da
NCBI Official Full Name
Carbonic anhydrase 2
NCBI Official Synonym Full Names
carbonic anhydrase 2
NCBI Official Symbol
Car2
NCBI Official Synonym Symbols
Ca2; CAII; Car-2; Ltw-5; Lvtw-5; AI131712
NCBI Protein Information
carbonic anhydrase 2
Protein Family
Research Articles on CA2
Similar Products
Product Notes
The CA2 (Catalog #AAA8122910) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Met1-Lys26 0. It is sometimes possible for the material contained within the vial of "Carbonic anhydrase II, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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