Typical Testing Data/Standard Curve
Mouse CD169 ELISA Kit | SIGLEC1 elisa kit
Mouse CD169 ELISA Kit
Gene Names
Siglec1; Sn; Cd169; Siglec-1
Reactivity
Mouse
Applications
ELISA
Synonyms
CD169; Mouse CD169 ELISA Kit; SN; Sialoadhesin; Sialic acid-binding Ig-like lectin 1; Siglec-1; SIGLEC1 elisa kit
Reactivity
Mouse
Specificity
The Mouse CD169 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse CD169 proteins within the range of 156 pg/ml - 10000 pg/ml.
Sequence Length
1695
Applicable Applications for SIGLEC1 elisa kit
Sandwich ELISA (EIA)
Samples
Cell Culture Supernatants, Serum, Plasma.
Sensitivity
10 pg/ml.
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve
Related Product Information for SIGLEC1 elisa kit
Principle of the Assay||The Mouse CD169 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse CD169 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Mouse CD169 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD169 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse CD169 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD169 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background/Introduction: SIGLEC-1, also known as Sialoadhesin or CD169, is a cell adhesion molecule found on the surface of certain cells of the immune system called macrophages. This gene is mapped to 20p13. It belongs to the immunoglobulin superfamily (IgSF). Since sialoadhesin binds sialic acids with its N-terminal IgV-domain, it is also a member of the SIGLEC family. The localization and expression of SIGLEC-1 in humans has altered coincident with the evolutionary loss of Neu5Gc. SIGLEC-1-positive macrophages have a dual physiologic function. They act as innate 'flypaper' by preventing the systemic spread of lymph-borne pathogens and as critical gatekeepers at the lymph-tissue interface that facilitate the recognition of particulate antigens by B cells and initiate humoral immune responses.
Background/Introduction: SIGLEC-1, also known as Sialoadhesin or CD169, is a cell adhesion molecule found on the surface of certain cells of the immune system called macrophages. This gene is mapped to 20p13. It belongs to the immunoglobulin superfamily (IgSF). Since sialoadhesin binds sialic acids with its N-terminal IgV-domain, it is also a member of the SIGLEC family. The localization and expression of SIGLEC-1 in humans has altered coincident with the evolutionary loss of Neu5Gc. SIGLEC-1-positive macrophages have a dual physiologic function. They act as innate 'flypaper' by preventing the systemic spread of lymph-borne pathogens and as critical gatekeepers at the lymph-tissue interface that facilitate the recognition of particulate antigens by B cells and initiate humoral immune responses.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
172,769 Da
NCBI Official Full Name
Sialoadhesin
NCBI Official Synonym Full Names
sialic acid binding Ig-like lectin 1, sialoadhesin
NCBI Official Symbol
Siglec1
NCBI Official Synonym Symbols
Sn; Cd169; Siglec-1
NCBI Protein Information
sialoadhesin
UniProt Protein Name
Sialoadhesin
Protein Family
UniProt Gene Name
Siglec1
UniProt Synonym Gene Names
Sa; Sn; SER; Siglec-1
UniProt Entry Name
SN_MOUSE
Uniprot Description
SIGLEC1: Acts as an endocytic receptor mediating clathrin dependent endocytosis. Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Preferentially binds to alpha-2,3-linked sialic acid. Binds to SPN/CD43 on T-cells. May play a role in hemopoiesis. Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Cellular Component: extracellular region; integral to membrane; membrane; plasma membrane
Molecular Function: carbohydrate binding; protein binding
Biological Process: cell adhesion; endocytosis
Research Articles on SIGLEC1
Similar Products
Product Notes
The Mouse SIGLEC1 siglec1 (Catalog #AAA8244707) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA8244707 ELISA Kit recognizes Mouse SIGLEC1. AAA Biotech's CD169 can be used in a range of immunoassay formats including, but not limited to, Sandwich ELISA (EIA). Researchers should empirically determine the suitability of the SIGLEC1 siglec1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD169, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
