Chromatin Immunoprecipitation (ChIP)
(EZH2 antibody (mAb) tested by ChIP. Chromatin IP performed using the ChIP-IT Express Kit and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 3 ug of EZH2 antibody or the equivalent amount of IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from the ChIP reaction using a primer pair specific for the Myt1 gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.)
Mouse anti-Human, Mouse EZH2 Monoclonal Antibody | anti-EZH2 antibody
EZH2 antibody (mAb)(Clone AC22)
Reactivity
Human, Mouse
Applications
Chromatin Immunoprecipitation, Immunoprecipitation
Purity
Protein A Chromatography
Synonyms
EZH2; Monoclonal Antibody; EZH2 antibody (mAb)(Clone AC22); WVS; ENX1; EZH1; KMT6; WVS2; ENX-1; EZH2b; KMT6A; anti-EZH2 antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
AC22
Purity/Purification
Protein A Chromatography
Form/Format
Purified IgG in 70mM Tris (pH8), 105mM NaCl, 31mM glycine, 0.07mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-EZH2 antibody
Chromatin Immunoprecipitation (ChIP)
Application Notes
Applications Validated:
ChIP: 1-3ug per ChIP
This antibody has been published, but not validated, for Western blot. For an antibody we have tested in use in Western blotting
ChIP: 1-3ug per ChIP
This antibody has been published, but not validated, for Western blot. For an antibody we have tested in use in Western blotting
Immunogen
This EZH2 antibody was raised against a recombinant protein corresponding to amino acids 353-451 of human EZH2.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Chromatin Immunoprecipitation (ChIP)
(EZH2 antibody (mAb) tested by ChIP. Chromatin IP performed using the ChIP-IT Express Kit and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 3 ug of EZH2 antibody or the equivalent amount of IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from the ChIP reaction using a primer pair specific for the Myt1 gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.)
Chromatin Immunoprecipitation (ChIP)
(EZH2 antibody (mAb) tested by ChIP. Chromatin IP performed using the ChIP-IT Express Kit and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 3 ug of EZH2 antibody or the equivalent amount of IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from the ChIP reaction using a primer pair specific for the Myt1 gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.)
Related Product Information for anti-EZH2 antibody
Short Description: Our EZH2 antibody (mAb)(Clone AC22) was raised in a Mouse host. It has been validated for use in Chromatin Immunoprecipitation; it has been shown to react with Human and Mouse samples.
Background: EZH2-(Enhancer of Zeste homolog 2, also designated Enx1 and SETDB1) is a human homolog of the Drosophila Polycomb-group protein Enhancer of Zeste protein. It contains a SET domain that catalyzes the methylation of histone H3 at lysine 27 (Histone H3 Lys27 monomethylated, dimethylated and trimethylated). Polycomb-group proteins repress gene expression by binding to chromatin and locally altering chromatin structure. EZH2, BMI-1 and Suz12 are present in the PRC2 and PRC3 protein complexes that function as mediators of epigenetic transcriptional silencing. EZH2 associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. EZH2 may play a role in the hematopoietic and central nervous systems. Deregulation of EZH2 is linked to a variety of cancers.
Background: EZH2-(Enhancer of Zeste homolog 2, also designated Enx1 and SETDB1) is a human homolog of the Drosophila Polycomb-group protein Enhancer of Zeste protein. It contains a SET domain that catalyzes the methylation of histone H3 at lysine 27 (Histone H3 Lys27 monomethylated, dimethylated and trimethylated). Polycomb-group proteins repress gene expression by binding to chromatin and locally altering chromatin structure. EZH2, BMI-1 and Suz12 are present in the PRC2 and PRC3 protein complexes that function as mediators of epigenetic transcriptional silencing. EZH2 associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. EZH2 may play a role in the hematopoietic and central nervous systems. Deregulation of EZH2 is linked to a variety of cancers.
Product Categories/Family for anti-EZH2 antibody
NCBI and Uniprot Product Information
Molecular Weight
96kDa
Protein Family
Similar Products
Product Notes
The EZH2 (Catalog #AAA388356) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The EZH2 antibody (mAb)(Clone AC22) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's EZH2 can be used in a range of immunoassay formats including, but not limited to, Chromatin Immunoprecipitation (ChIP). Applications Validated: ChIP: 1-3ug per ChIP This antibody has been published, but not validated, for Western blot. For an antibody we have tested in use in Western blotting. Researchers should empirically determine the suitability of the EZH2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EZH2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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