Carbonic Anhydrase IX Active Protein | CA9 active protein

Carbonic Anhydrase IX Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122900

• Gene Names: Car9; Ca9; CAIX; MN/CA9
• Purity: >95% as determined by SDS-PAGE
• Synonyms: Carbonic Anhydrase IX; Carbonic Anhydrase IX Protein; Mouse; Recombinant (His Tag); Mouse Carbonic Anhydrase IX/Car9 Protein (His Tag); carbonic anhydrase IX; CA9 active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Asp390

• Species: Mouse
• Activity: Measured by its esterase activity. The specific activity is >100pmoles/min/ug.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gln 32
• Tag: C-His
• Protein Construction: A DNA sequence encoding the extracellular domain of mouse CAR9 (NP_647466.2)(Met 1-Asp 390) was expressed, with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CA9 active protein

Background: Carbonic anhydrases IX (CA IX), also known as membrane antigen MN or CA9, is a member of the carbonic anhydrase (CA) family and may be involved in cell proliferation and cellular transformation. CAs are zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide (H2O + CO2 = H+ + HCO3-) and thus participate in a variety of biological and physical processes. CA IX protein is expressed primarily in carcinoma cells lines, and the expression is cell density dependent and has been shown to be strongly induced by hypoxia, accordingly facilitates adaptation of tumor cells to hypoxic conditions. It is involved in tumorigenesis through many pathways, such as pH regulation and cell adhesion control. CA IX is used as a marker of tumor hypoxia and as a new therapeutic target for many human carcinomas and cancers.

Product Categories/Family for CA9 active protein

Cancer Drug Targets

References

Loncaster JA, et al. (2001) Carbonic anhydrase (CA IX) expression, a potential new intrinsic marker of hypoxia: correlations with tumor oxygen measurements and prognosis in locally advanced carcinoma of the cervix. Cancer Res. 61(17): 6394-9.
Zvada J, et al. (2003) Soluble form of carbonic anhydrase IX (CA IX) in the serum and urine of renal carcinoma patients. Br J Cancer. 89(6): 1067-71.
Pan P, et al. (2006) Carbonic anhydrase gene expression in CA II-deficient (Car2-/-) and CA IX-deficient (Car9-/-) mice. J Physiol. 571(Pt 2): 319-27.
Zhou GX, et al. (2010) Quantification of carbonic anhydrase IX expression in serum and tissue of renal cell carcinoma patients using enzyme-linked immunosorbent assay: prognostic and diagnostic potentials. Urology. 75(2): 257-61.

NCBI and Uniprot Product Information

• NCBI GI #: 145046220
• NCBI GeneID: 230099
• NCBI Accession #: NP_647466.2
• NCBI GenBank Nucleotide #: NM_139305.2
• UniProt Accession #: Q8VHB5; Q8K1G1; Q8VDE4
• Molecular Weight: 30,247 Da
• NCBI Official Full Name: carbonic anhydrase 9
• NCBI Official Synonym Full Names: carbonic anhydrase 9
• NCBI Official Symbol: Car9
• NCBI Official Synonym Symbols: Ca9; CAIX; MN/CA9
• NCBI Protein Information: carbonic anhydrase 9
• UniProt Protein Name: Carbonic anhydrase 9
• Protein Family: Carbonic anhydrase
• UniProt Gene Name: Ca9
• UniProt Synonym Gene Names: Car9; CA-IX; CAIX

Uniprot Description

CA9: Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia. Forms oligomers linked by disulfide bonds. By hypoxia. Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa. Inhibited by coumarins, saccharin, sulfonamide derivatives such as acetazolamide (AZA) and Foscarnet (phosphonoformate trisodium salt). Belongs to the alpha-carbonic anhydrase family.

Protein type: EC 4.2.1.1; Energy Metabolism - nitrogen; Lyase; Membrane protein, integral; Nucleolus

Chromosomal Location of Human Ortholog: 4|4 A5

Cellular Component: basolateral plasma membrane; plasma membrane

Biological Process: morphogenesis of an epithelium; secretion