Western Blot (WB)
(Anti-CA10 rabbit polyclonal antibody at 1:500 dilutionLane A: HL-60 Whole Cell LysateLane B: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:38 kDaObserved band size:41 kDa(We are unsure as to the identity of these extra bands.))
Rabbit anti-Mouse Carbonic Anhydrase X Polyclonal Antibody | anti-CA10 antibody
Anti-Carbonic Anhydrase X Antibody, Rabbit Polyclonal
Gene Names
Car10; Ca10; BB085816; 2700029L05Rik
Reactivity
Mouse
Applications
Western Blot, ELISA
Purity
Protein A & Antigen Affinity
Synonyms
Carbonic Anhydrase X; Polyclonal Antibody; Anti-Carbonic Anhydrase X Antibody; Rabbit Polyclonal; Carbonic Anhydrase X/Car10 Antibody; Rabbit PAb; Antigen Affinity Purified; carbonic anhydrase X; Anti-2700029L05Rik Antibody; Anti-BB085816 Antibody; Anti-Ca10 Antibody; Anti-Car10 Antibody; Anti-RP23-448I9.1 Antibody; anti-CA10 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Mouse Carbonic Anhydrase X/CA10
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-CA10 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
ELISA: 1:5000-1:10,000
ELISA: 1:5000-1:10,000
Immunogen
Recombinant Mouse Carbonic Anhydrase X/CA10 protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Mouse Carbonic Anhydrase X/CA10 (rM Carbonic Anhydrase X/CA10). Carbonic Anhydrase X/CA10 specific IgG was purified by Mouse Carbonic Anhydrase X/CA10 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-CA10 rabbit polyclonal antibody at 1:500 dilutionLane A: HL-60 Whole Cell LysateLane B: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:38 kDaObserved band size:41 kDa(We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-CA10 rabbit polyclonal antibody at 1:500 dilutionLane A: HL-60 Whole Cell LysateLane B: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:38 kDaObserved band size:41 kDa(We are unsure as to the identity of these extra bands.))
Related Product Information for anti-CA10 antibody
Carbonic anhydrase X, also called carbonic anhydrase-related protein X (CARPX) and CA1, belongs to the CA family of zinc metalloenzymes which catalyze the reversible hydration of carbon dioxide in various biological processes such as respiration, renal tubular acidification and bone resorption. The secreted protein CARPX without CA activity (hydration of CO2) is identified as an acatalytic member of the alpha-carbonic anhydrase subgroup. CARP X expression is detected in the adult total brain and almost all parts of the central nervous system, but not in the fetal brain. Accordingly, CARP X is suggested to play a role in the development of central nervous system, especially the brain. The same CARP X protein are encoded by multiple transcript variants of this gene.
References
Okamoto, N. et al., 2001, Biochim. Biophys. Acta 1518:311-316. Taniuchi, K. et al., Neuroscience 112: 93-99. Supuran, C.T. et al., 2003, Med. Res. Rev. 23: 146-189.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
37,563 Da
NCBI Official Full Name
carbonic anhydrase-related protein 10
NCBI Official Synonym Full Names
carbonic anhydrase 10
NCBI Official Symbol
Car10
NCBI Official Synonym Symbols
Ca10; BB085816; 2700029L05Rik
NCBI Protein Information
carbonic anhydrase-related protein 10
UniProt Protein Name
Carbonic anhydrase-related protein 10
Protein Family
UniProt Gene Name
Ca10
UniProt Synonym Gene Names
Car10; CA-RP X; CARP X
Uniprot Description
CA10: Does not have a catalytic activity. Belongs to the alpha-carbonic anhydrase family.
Chromosomal Location of Human Ortholog: 11|11 D
Research Articles on CA10
Similar Products
Product Notes
The CA10 ca10 (Catalog #AAA8112048) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Carbonic Anhydrase X Antibody, Rabbit Polyclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Carbonic Anhydrase X can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:2000 ELISA: 1:5000-1:10,000. Researchers should empirically determine the suitability of the CA10 ca10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Carbonic Anhydrase X, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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