PRSS2 active protein

PRSS2 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122712

• Gene Names: Prss2; Ta; TRY8; TRYP; Try2; Tesp4
• Purity: >92% as determined by SDS-PAGE
• Synonyms: PRSS2; PRSS2 Protein; Mouse; Recombinant (His Tag); Mouse Trypsin 2/PRSS2 Protein (His Tag); Prss2 Protein; Ta Protein; Tesp4 Protein; Try2 Protein; TRY8 Protein; TRYP Protein; protease; serine 2; PRSS2 active protein

• Host: HEK293 Cells
• Purity/Purification: >92% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Asn246

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Phe 16
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse PRSS2 (NP_033456.1) precursor (Met 1-Asn 246) was expressed, with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. The specific activity is >1500pmoles/min/ug. (Activation description: The proenzyme needs to be activated by enteropeptidase for an activated form)
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for PRSS2 active protein

Background: Trypsin-2, also known as Trypsin II, Anionic trypsinogen, Serine protease 2, PRSS2 and TRY2, is a secreted protein that belongs to the trypsin serine protease family including Trypsin, PRSS1, PRSS2 and PRSS3. It consists of a signal peptide (residues 1-15), a pro region (residues 16-23), and a proteolytically active mature chain (residues 24-247). PRSS2 contains one peptidase S1 domain. It is secreted into the duodenum, hydrolysing peptides into their smaller building blocks, which is necessary for the uptake of protein in the food. It is secreted by the pancreas in the form of inactive zymogen, trypsinogen and cleaved to its active form in the small intestine when the pancreas is stimulated by cholecystokinin through the common activation mechanism.

Product Categories/Family for PRSS2 active protein

Serine Proteases and Regulators

References

Rawlings, N.D. et al.,1994, Meth. Enzymol. 244: 19-61. Noone, P.G. et al., 2001, Gastroenterology. 121 (6): 1310-9. Leiros, H.K. et al., 2004, Protein Sci. 13 (4): 1056-70. Ra³nai,Z. et al., 2009, Biochem J. 418 (1):155-61.

NCBI and Uniprot Product Information

• NCBI GI #: 6678439
• NCBI GeneID: 22072
• NCBI Accession #: NP_033456.1
• NCBI GenBank Nucleotide #: NM_009430.2
• UniProt Accession #: P07146
• Molecular Weight: 26,204 Da
• NCBI Official Full Name: anionic trypsin-2
• NCBI Official Synonym Full Names: protease, serine 2
• NCBI Official Symbol: Prss2
• NCBI Official Synonym Symbols: Ta; TRY8; TRYP; Try2; Tesp4
• NCBI Protein Information: anionic trypsin-2
• UniProt Protein Name: Anionic trypsin-2
• Protein Family: Trypsin
• UniProt Gene Name: Prss2
• UniProt Synonym Gene Names: Try2

Uniprot Description

trypsin 2: In the ileum, may be involved in defensin processing, including DEFA5. Belongs to the peptidase S1 family.

Protein type: Calcium-binding; Cell adhesion; EC 3.4.21.4; Extracellular matrix; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 6|6 B1

Cellular Component: extracellular region; extracellular space

Molecular Function: calcium ion binding; hydrolase activity; metal ion binding; peptidase activity; serine-type endopeptidase activity; serine-type peptidase activity

Biological Process: collagen catabolic process; digestion; proteolysis; response to caffeine; response to nicotine