Rabbit FURIN Antibody | anti-FURIN antibody

FURIN

Cat. Num. AAA9127539

• Gene Names: FURIN; FUR; PACE; SPC1; PCSK3
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity purification
• Synonyms: FURIN; FUR; PACE; PCSK3; SPC1; furin; anti-FURIN antibody

• Host: Rabbit
• Isotype: IgG
• Purity/Purification: Affinity purification
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

• Applicable Applications for anti-FURIN antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:200
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 120-370 of human FURIN (NP_002560.1).
• Species Reactivity/Cross-Reactivity: Human, Mouse, Rat
• Preparation and Storage: Store at -20°C. Avoid freeze / thaw cycles

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using FURIN antibody (MBS9127539) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human liver cancer using FURIN antibody (MBS9127539) at dilution of 1:100 (40xlens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of A549 cells using FURIN Rabbit pAb (MBS9127539) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.)

Immunofluorescence (IF)

(Immunofluorescence analysis of NIH-3T3 cells using FURIN Rabbit pAb (MBS9127539) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.)

Immunofluorescence (IF)

(Immunofluorescence analysis of U-2 OS cells using FURIN Rabbit pAb (MBS9127539) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.)

Related Product Information for anti-FURIN antibody

This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. It encodes a type 1 membrane bound protease that is expressed in many tissues, including neuroendocrine, liver, gut, and brain. The encoded protein undergoes an initial autocatalytic processing event in the ER and then sorts to the trans-Golgi network through endosomes where a second autocatalytic event takes place and the catalytic activity is acquired. The product of this gene is one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. Some of its substrates include proparathyroid hormone, transforming growth factor beta 1 precursor, proalbumin, pro-beta-secretase, membrane type-1 matrix metalloproteinase, beta subunit of pronerve growth factor and von Willebrand factor. It is also thought to be one of the proteases responsible for the activation of HIV envelope glycoproteins gp160 and gp140 and may play a role in tumor progression. This gene is located in close proximity to family member proprotein convertase subtilisin/kexin type 6 and upstream of the FES oncogene. Alternative splicing results in multiple transcript variants.

Product Categories/Family for anti-FURIN antibody

Polyclonal

NCBI and Uniprot Product Information

• NCBI GI #: 120611
• NCBI GeneID: 5045
• UniProt Accession #: P09958; Q14336; Q6LBS3; Q9UCZ5
• Molecular Weight: Observed MW: 90KDa; Calculated MW: 86kDa
• NCBI Official Full Name: Furin
• NCBI Official Synonym Full Names: furin (paired basic amino acid cleaving enzyme)
• NCBI Official Symbol: FURIN
• NCBI Official Synonym Symbols: FUR; PACE; SPC1; PCSK3
• NCBI Protein Information: furin; FES upstream region; dibasic processing enzyme; furin, membrane associated receptor protein; proprotein convertase subtilisin/kexin type 3; paired basic amino acid residue-cleaving enzyme
• UniProt Protein Name: Furin
• Protein Family: Furin
• UniProt Gene Name: FURIN
• UniProt Synonym Gene Names: FUR; PACE; PCSK3; PACE
• UniProt Entry Name: FURIN_HUMAN

NCBI Description

This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. It encodes a type 1 membrane bound protease that is expressed in many tissues, including neuroendocrine, liver, gut, and brain. The encoded protein undergoes an initial autocatalytic processing event in the ER and then sorts to the trans-Golgi network through endosomes where a second autocatalytic event takes place and the catalytic activity is acquired. The product of this gene is one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. Some of its substrates include proparathyroid hormone, transforming growth factor beta 1 precursor, proalbumin, pro-beta-secretase, membrane type-1 matrix metalloproteinase, beta subunit of pro-nerve growth factor and von Willebrand factor. It is also thought to be one of the proteases responsible for the activation of HIV envelope glycoproteins gp160 and gp140 and may play a role in tumor progression. This gene is located in close proximity to family member proprotein convertase subtilisin/kexin type 6 and upstream of the FES oncogene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

FURIN: Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif. Belongs to the peptidase S8 family. Furin subfamily.

Protein type: EC 3.4.21.75; Protease; Cell surface; Vesicle; Membrane protein, integral

Chromosomal Location of Human Ortholog: 15q26.1

Cellular Component: Golgi membrane; extracellular space; trans-Golgi network transport vesicle; cell surface; membrane; Golgi lumen; integral to membrane; plasma membrane; trans-Golgi network; lipid raft

Molecular Function: peptidase activity; serine-type endopeptidase inhibitor activity; protease binding; serine-type endopeptidase activity; metal ion binding; endopeptidase activity; nerve growth factor binding; peptide binding

Biological Process: extracellular matrix organization and biogenesis; nerve growth factor receptor signaling pathway; viral reproduction; peptide hormone processing; regulation of signal transduction; pathogenesis; viral infectious cycle; proteolysis; positive regulation of membrane protein ectodomain proteolysis; peptide biosynthetic process; extracellular matrix disassembly; nerve growth factor processing; transforming growth factor beta receptor signaling pathway; regulation of endopeptidase activity; negative regulation of nerve growth factor production; secretion by cell; Notch signaling pathway; signal peptide processing; virus assembly; post-translational protein modification; peptidyl-glutamic acid carboxylation; nerve growth factor production; negative regulation of transforming growth factor-beta1 production; collagen catabolic process; cell proliferation; cellular protein metabolic process; viral protein processing; regulation of protein catabolic process; protein processing; negative regulation of low-density lipoprotein receptor catabolic process