Kallikrein 7/KLK7 Active Protein | KLK7 active protein

Kallikrein 7/KLK7 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8123031

• Gene Names: Klk7; SCCE; Prss6
• Purity: >95% as determined by SDS-PAGE
• Synonyms: Kallikrein 7/KLK7; Kallikrein 7/KLK7 Protein; Mouse; Recombinant (His Tag); Mouse KLK7/Kallikrein 7 Protein (His Tag); Prss6 Protein; SCCE Protein; kallikrein-related peptidase 7; KLK7 active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Arg249

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gln 22
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse KLK7 (Q91VE3)(Met1-Arg249) was expressed with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. The specific activity is >70pmoles/min/ug. (Activation description: The proenzyme needs to be activated by Thermolysin for an activated form)
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for KLK7 active protein

Background: Kallikrein-7, also known as kallikrein-related peptidase 7, Stratum corneum chymotryptic enzyme, Serine protease 6, KLK7, and PRSS6, is a secreted protein that belongs to the peptidase S1 family and Kallikrein subfamily. Members of the Kallikrein family are involved in various malignancies such as prostate (PSA, KLK2, KLK15), ovarian (KLK4, KLK5, KLK6, KLK8, KLK1), and breast cancer (KLK1, KLK13, KLK14). Kallikrein-7/KLK7 appears to be increased in ovarian cancer and higher KLK7 expression in ovarian cancer tissue is associated with poorer prognosis of ovarian cancer patients. Kallikrein-7/KLK7 is abundantly expressed in the skin and is expressed by keratinocytes in the epidermis. Kallikrein-7/KLK7 is up-regulated in ovarian carcinoma, especially late-stage serous carcinoma, compared with normal ovaries and benign adenomas (at the protein level). It was significantly associated with shorter overall survival (OS) and disease-free survival (DFS). Kallikrein-7/KLK7 may catalyze the degradation of intercellular cohesive structures in the cornified layer of the skin in the continuous shedding of cells from the skin surface. KLK7 also plays a role in the activation of precursors to inflammatory cytokines.

Product Categories/Family for KLK7 active protein

Serine Proteases and Regulators

References

Hansson L., et al.,(1994), Cloning, expression, and characterization of stratum corneum chymotryptic enzyme. A skin-specific human serine proteinase. J. Biol. Chem. 269:19420-19426.Yousef G.M., et al., (2000), The KLK7 (PRSS6) gene, encoding for the stratum corneum chymotryptic enzyme is a new member of the human kallikrein gene family -- genomic characterization, mapping, tissue expression and hormonal regulation.Gene 254:119-128.Gan L., et al.,(2000), Sequencing and expression analysis of the serine protease gene cluster located in chromosome 19q13 region.Gene 257:119-130.

NCBI and Uniprot Product Information

• NCBI GI #: 18777665
• NCBI GeneID: 23993
• NCBI Accession #: NP_036002.1
• NCBI GenBank Nucleotide #: NM_011872.3
• UniProt Accession #: Q91VE3; Q9R048
• Molecular Weight: 27,257 Da
• NCBI Official Full Name: kallikrein-7 preproprotein
• NCBI Official Synonym Full Names: kallikrein related-peptidase 7 (chymotryptic, stratum corneum)
• NCBI Official Symbol: Klk7
• NCBI Official Synonym Symbols: SCCE; Prss6
• NCBI Protein Information: kallikrein-7
• UniProt Protein Name: Kallikrein-7
• Protein Family: Kallikrein
• UniProt Gene Name: Klk7
• UniProt Synonym Gene Names: Prss6; Scce

NCBI Description

This gene encodes a member of the kallikrein subfamily of serine proteases that are involved in diverse physiological functions such as skin desquamation, tooth enamel formation, seminal liquefaction, synaptic neural plasticity and brain function. The encoded preproprotein undergoes proteolytic cleavage of the activation peptide to generate the functional enzyme. This gene is located in a cluster of several related kallikrein genes on chromosome 7. [provided by RefSeq, May 2016]

Uniprot Description

May catalyze the degradation of intercellular cohesive structures in the cornified layer of the skin in the continuous shedding of cells from the skin surface. Specific for amino acid residues with aromatic side chains in the P1 position. Cleaves insulin A chain at '14-Tyr-|-Gln-15' and insulin B chain at '6-Leu-|-Cys-7', '16-Tyr-|-Leu-17', '25-Phe-|-Tyr-26' and '26-Tyr-|-Thr-27'. Could play a role in the activation of precursors to inflammatory cytokines.