SDS-Page
PRSS3 active protein
PRSS3 Protein, Human, Recombinant (His Tag)
Gene Names
PRSS3; T9; MTG; TRY3; TRY4; PRSS4
Purity
>95% as determined by SDS-PAGE
Synonyms
PRSS3; PRSS3 Protein; Human; Recombinant (His Tag); Human Trypsin-3/PRSS3 Protein (His Tag); MTG Protein; PRSS4 Protein; RP11-176F3.3 Protein; T9 Protein; TRY3 Protein; TRY4 Protein; protease; serine 3; PRSS3 active protein
Host
HEK293 Cells
Purity/Purification
>95% as determined by SDS-PAGE
Form/Format
Lyophilized from sterile 50mM MES, 0.6M NaCl, pH5.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Sequence
Met1-Ser247
Species
Human
Endotoxin
<1.0EU per ug of the protein as determined by the LAL method
Predicted N Terminal
Val 16
Tag
C-His
Protein Construction
A DNA sequence encoding the human PRSS3 isoform c (P35030-3)(Met 1-Ser 247) was expressed, with a polyhistidine tag at the C-terminus.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Activity
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. The specific activity is >4,000pmoles/min/ug. (Activation description: The proenzyme needs to be activated by enteropeptidase for an activated form)
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
SDS-Page
SDS-Page
Related Product Information for PRSS3 active protein
Background: Trypsin-3, also known as Trypsin III, brain trypsinogen, Serine protease 3 and PRSS3, is a secreted protein that belongs to the peptidase S1 family. Trypsin-3/PRSS3 is expressed is in pancreas and brain. It contains one peptidase S1 domain. Trypsin-3/PRSS3 can degrade intrapancreatic trypsin inhibitors that protect against CP. Genetic variants that cause higher mesotrypsin activity might increase the risk for chronic pancreatitis (CP). A sustained imbalance of pancreatic proteases and their inhibitors seems to be important for the development of CP. The trypsin inhibitor-degrading activity qualified PRSS3 as a candidate for a novel CP susceptibility gene. Trypsin-3/PRSS3 has been implicated as a putative tumor suppressor gene due to its loss of expression, which is correlated with promoter hypermethylation, in esophageal squamous cell carcinoma and gastric adenocarcinoma.
Product Categories/Family for PRSS3 active protein
References
Venter JC. et al., 2001, Science 291:1304-51. Marsit,CJ. et al., 2005, Mol Carcinog 44 (2):146-50. Rowen, L. et al., 2005, Mol Biol Evol. 22 (8):1712-20. Rosendahl, J. et al., 2010, Pancreatology. 10 (2-3):243-9
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
25,924 Da
NCBI Official Full Name
trypsin-3 isoform 3 preproprotein
NCBI Official Synonym Full Names
protease, serine 3
NCBI Official Symbol
PRSS3
NCBI Official Synonym Symbols
T9; MTG; TRY3; TRY4; PRSS4
NCBI Protein Information
trypsin-3
UniProt Protein Name
Trypsin-3
UniProt Gene Name
PRSS3
UniProt Synonym Gene Names
PRSS4; TRY3; TRY4
UniProt Entry Name
TRY3_HUMAN
Uniprot Description
Digestive protease specialized for the degradation of trypsin inhibitors. In the ileum, may be involved in defensin processing, including DEFA5.
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Product Notes
The PRSS3 prss3 (Catalog #AAA8121002) is an Active Protein produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Met1-Ser24 7. It is sometimes possible for the material contained within the vial of "PRSS3, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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