Rabbit DAAM1 Polyclonal Antibody | anti-DAAM1 antibody

DAAM1-T361, NT (DAAM1, KIAA0666, Disheveled-associated activator of morphogenesis 1) (APC)

Cat. Num. AAA6290662

• Reactivity: Hamster, Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DAAM1; Polyclonal Antibody; DAAM1-T361; NT (DAAM1; KIAA0666; Disheveled-associated activator of morphogenesis 1) (APC); Disheveled-associated activator of morphogenesis 1; anti-DAAM1 antibody

• Host: Rabbit
• Reactivity: Hamster, Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-DAAM1 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: DAAM1-T361 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 348-378 amino acids from the N-terminal region of human DAAM1-T361.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(DAAM1-T361 Antibody (N-term) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the DAAM1 antibody detected the DAAM1 protein (arrow).)

Western Blot (WB)

(DAAM1-T361 Antibody (N-term) western blot analysis in CHO cell line lysates (35ug/lane).This demonstrates the DAAM1 antibody detected the DAAM1 protein (arrow).)

Immunohistochemistry (IHC)

(DAAM1-T361 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DAAM1-T361 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(DAAM1-T361 Antibody (N-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-DAAM1 antibody

Functions of the cell cortex, including motility, adhesion, and cytokinesis, are mediated by the reorganization of the actin cytoskeleton and recent evidence suggests a role for the Formin homology (FH) proteins in these processes. The protein encoded by this gene contains FH domains and belongs to a novel FH protein subfamily implicated in cell polarity. Wnt/Fz signaling activates the small GTPase Rho, a key regulator of cytoskeleton architecture, to control cell polarity and movement during development. Activation requires Dvl-Rho complex formation, an assembly mediated by this gene product, which is thought to function as a scaffolding protein. Evidence of alternative splicing has been observed for this gene but the full-length nature of these variants has not been determined.

Product Categories/Family for anti-DAAM1 antibody

Antibodies; KIAA; orf

References

Higashi, T., et al. J. Biol. Chem. 283(13):8746-8755(2008). Liu, W., et al. Proc. Natl. Acad. Sci. U.S.A. 105(1):210-215(2008). Yamashita, M., et al. Genes Cells 12(11):1255-1265(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 21071077
• NCBI GeneID: 23002
• NCBI Accession #: NP_055807.1
• NCBI GenBank Nucleotide #: NM_014992.2
• UniProt Accession #: Q9Y4D1; Q86U34; Q8N1Z8; Q8TB39
• Molecular Weight: 100,745 Da
• NCBI Official Full Name: disheveled-associated activator of morphogenesis 1 isoform 1
• NCBI Official Synonym Full Names: dishevelled associated activator of morphogenesis 1
• NCBI Official Symbol: DAAM1
• NCBI Protein Information: disheveled-associated activator of morphogenesis 1
• UniProt Protein Name: Disheveled-associated activator of morphogenesis 1
• Protein Family: Disheveled-associated activator of morphogenesis
• UniProt Gene Name: DAAM1
• UniProt Synonym Gene Names: KIAA0666
• UniProt Entry Name: DAAM1_HUMAN

NCBI Description

Cell motility, adhesion, cytokinesis, and other functions of the cell cortex are mediated by reorganization of the actin cytoskeleton and several formin homology (FH) proteins have been associated with these processes. The protein encoded by this gene contains two FH domains and belongs to a novel FH protein subfamily implicated in cell polarity. A key regulator of cytoskeletal architecture, the small GTPase Rho, is activated during development by Wnt/Fz signaling to control cell polarity and movement. The protein encoded by this gene is thought to function as a scaffolding protein for the Wnt-induced assembly of a disheveled (Dvl)-Rho complex. This protein also promotes the nucleation and elongation of new actin filaments and regulates cell growth through the stabilization of microtubules. Alternative splicing results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, Jul 2012]

Uniprot Description

DAAM1: Binds to disheveled (Dvl) and Rho, and mediates Wnt- induced Dvl-Rho complex formation. May play a role as a scaffolding protein to recruit Rho-GDP and Rho-GEF, thereby enhancing Rho-GTP formation. Can direct nucleation and elongation of new actin filaments. Belongs to the formin homology family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 14q23.1

Cellular Component: membrane; cytoplasm; stress fiber; plasma membrane; cytosol

Molecular Function: identical protein binding; protein binding; Rho GTPase binding; actin binding

Biological Process: actin cytoskeleton organization and biogenesis