Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Trypsin-2 (PRSS2) Standard Curve.)
Mouse Trypsin-2 (PRSS2) ELISA Kit | TRY2 elisa kit
Mouse Trypsin-2 (PRSS2) ELISA Kit
Gene Names
Prss2; Ta; TRY8; TRYP; Try2; Tesp4
Reactivity
Mouse
Synonyms
Trypsin-2 (PRSS2); Mouse Trypsin-2 (PRSS2) ELISA Kit; PRSS2; MGC111183; MGC120174; TRY8; TRYP2; anionic trypsinogen; protease serine 2 preproprotein; protease; serine; 2; preproprotein; trypsin 2; trypsin; trypsinogen 2; TRY2 elisa kit
Reactivity
Mouse
Specificity
Mouse Trypsin-2 (PRSS2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PRSS2. No significant cross-reactivity or interference between Mouse PRSS2 and analogues was observed.
Sequence Length
246
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Trypsin-2 (PRSS2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Trypsin-2 (PRSS2) Standard Curve.)
Related Product Information for TRY2 elisa kit
Description: This Mouse Trypsin-2 (PRSS2) ELISA Kit employs a two-site sandwich ELISA to quantitate PRSS2 in samples. An antibody specific for PRSS2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRSS2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRSS2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRSS2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: In the ileum, PRSS2 may be involved in defensin processing, including DEFA5. PRSS2 is a member of the trypsin family of serine proteases. This enzyme is secreted by the pancreas and cleaved to its active form in the small intestine. It is active on peptide linkages involving the carboxyl group of lysine or arginine. This gene and several other trypsinogen genes are localized to the T cell receptor beta locus on chromosome 7. Trypsin is a serine protease found in the digestive system of many vertebrates, where it hydrolyses proteins.Rowen et al. (1996) mapped the gene corresponding to the third pancreatic trypsinogen cDNA by fluorescence in situ hybridization. They used a cosmid clone containing 3 trypsinogen genes. Strong hybridization to chromosome 7 and weaker hybridization to chromosome 9 were observed. They isolated and partially sequenced 4 cosmid clones from the chromosome 9 region. They found that the region represents a duplication and translocation of a DNA segment from the 3-prime end of the TCRB locus that includes at least 7 V(beta) elements and a functional trypsinogen gene denoted T9.
Background: In the ileum, PRSS2 may be involved in defensin processing, including DEFA5. PRSS2 is a member of the trypsin family of serine proteases. This enzyme is secreted by the pancreas and cleaved to its active form in the small intestine. It is active on peptide linkages involving the carboxyl group of lysine or arginine. This gene and several other trypsinogen genes are localized to the T cell receptor beta locus on chromosome 7. Trypsin is a serine protease found in the digestive system of many vertebrates, where it hydrolyses proteins.Rowen et al. (1996) mapped the gene corresponding to the third pancreatic trypsinogen cDNA by fluorescence in situ hybridization. They used a cosmid clone containing 3 trypsinogen genes. Strong hybridization to chromosome 7 and weaker hybridization to chromosome 9 were observed. They isolated and partially sequenced 4 cosmid clones from the chromosome 9 region. They found that the region represents a duplication and translocation of a DNA segment from the 3-prime end of the TCRB locus that includes at least 7 V(beta) elements and a functional trypsinogen gene denoted T9.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
anionic trypsin-2
NCBI Official Synonym Full Names
protease, serine 2
NCBI Official Symbol
Prss2
NCBI Official Synonym Symbols
Ta; TRY8; TRYP; Try2; Tesp4
NCBI Protein Information
anionic trypsin-2
UniProt Protein Name
Anionic trypsin-2
Protein Family
UniProt Gene Name
Prss2
UniProt Synonym Gene Names
Try2
Uniprot Description
trypsin 2: In the ileum, may be involved in defensin processing, including DEFA5. Belongs to the peptidase S1 family.
Protein type: Calcium-binding; Cell adhesion; EC 3.4.21.4; Extracellular matrix; Protease; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 6|6 B1
Cellular Component: extracellular region; extracellular space
Molecular Function: calcium ion binding; hydrolase activity; metal ion binding; peptidase activity; serine-type endopeptidase activity; serine-type peptidase activity
Biological Process: collagen catabolic process; digestion; proteolysis; response to caffeine; response to nicotine
Research Articles on TRY2
Similar Products
Product Notes
The Mouse TRY2 prss2 (Catalog #AAA9717248) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717248 ELISA Kit recognizes Mouse TRY2. It is sometimes possible for the material contained within the vial of "Trypsin-2 (PRSS2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
