FYN recombinant protein

Recombinant FYN (2-537) protein

Cat. Num. AAA389412

• Synonyms: FYN; Recombinant FYN (2-537) protein; FYN Proto-Oncogene; Src Family Tyrosine Kinase); FYN recombinant protein

• Host: Baculovirus
• Form/Format: Recombinant FYN (2-537) protein is supplied in 25mM HEPES-NaOH pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100, and 0.5mM TCEP.

• Protein Species: Human
• Tag: DYKDDDDK-Tag
• Protein Details: Recombinant FYN (2-537) protein that includes amino acids 2-537 of human FYN protein (accession number NP_001357458.1) was expressed in a baculovirus expression system, and contains an N-terminal DYKDDDDK tag.
• Notes: This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant FYN (2-537) protein 10% SDS-PAGE gel with Coomassie blue staining MW: 61.6 kDa Purity: >95%)

Testing Data

(HTRF assay for FYN (2-537) activity 1 uM TK substrate was incubated with different concentrations of FYN (2-537) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection)

Testing Data

(HTRF assay for FYN (2-537) activity 1 uM TK substrate was incubated with different concentrations of FYN (2-537) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection)

Related Product Information for FYN recombinant protein

Background: FYN (FYN Proto-Oncogene, Src Family Tyrosine Kinase) is a non-receptor tyrosine-protein kinase that plays a role in many biological processes including regulation of cell growth and survival, cell adhesion, integrin-mediated signaling, cytoskeletal remodeling, cell motility, immune response and axon guidance. Inactive FYN is phosphorylated on its C-terminal tail within the catalytic domain. Following activation by PKA, the protein subsequently associates with PTK2/ FAK1, allowing PTK2/FAK1 phosphorylation, activation and targeting to focal adhesions. FYN involved in the regulation of cell adhesion and motility through phosphorylation of CTNNB1 (beta-catenin) and CTNND1 (delta-catenin) and regulates cytoskeletal remodeling by phosphorylating several proteins including the actin regulator WAS and the microtubuleassociated proteins MAP2 and MAPT. It also promotes cell survival by phosphorylating AGAP2/PIKE-A and preventing its apoptotic cleavage. Disieases associated with FYN include Hepatitis E and Autoimmune Cardiomyopathy

Product Categories/Family for FYN recombinant protein

Recombinant Proteins; Kinase

NCBI and Uniprot Product Information

• Molecular Weight: Molecular weight of the protein is 61.6kDa
• Protein Family: FYN-binding protein