JAK1 recombinant protein

Recombinant JAK1 (438-1154) protein

Cat. Num. AAA389413

• Gene Names: JAK1; JTK3; JAK1A; JAK1B
• Synonyms: JAK1; Recombinant JAK1 (438-1154) protein; Janus Kinase 1; JAK1 recombinant protein

• Host: Baculovirus
• Form/Format: Recombinant JAK1 (438-1154) protein is supplied in 25mM HEPESNaOH pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100, and 0.5mM TCEP.

• Protein Species: Human
• Tag: DYKDDDDK-Tag
• Protein Details: Recombinant JAK1 (438-1154) protein that includes amino acids 438-1154 of human JAK1 protein (accession number NP_001307852.1) was expressed in a baculovirus expression system, and contains an N-terminal DYKDDDDK tag.
• Notes: This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant JAK1 (438-1154) protein 10% SDS-PAGE gel with Coomassie blue staining MW: 83.6 kDa Purity: >65%)

Testing Data

(HTRF assay for JAK1 (438-1154) activity 1 uM TK substrate was incubated with different concentrations of JAK1 (438-1154) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM MnCl2, 1 mM DTT, and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)

Related Product Information for JAK1 recombinant protein

Background: JAK1 (Janus Kinase 1) is a non-receptor tyrosine kinase which is a member of a class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. JAK1 phosphorylates STAT proteins (signal transducers and activators of transcription) and plays a key role in interferon-alpha/beta, interferon-gamma, and cytokine signal transduction. It plays a crucial role in effecting the expression of genes that mediate inflammation, epithelial remodeling, and metastatic cancer progression It is a key component of the interleukin-6 (IL-6)/JAK1/STAT3 immune and inflammation response and a therapeutic target for alleviating cytokine storms. The kinase activity of this gene is directly inhibited by the suppressor of cytokine signalling 1 (SOCS1) protein. Diseases associated with JAK1 include Autoinflammation, Immune Dysregulation, And Eosinophilia and Autosomal Recessive Mendelian Susceptibility To Mycobacterial Diseases Due To Partial Jak1 Deficiency.

Product Categories/Family for JAK1 recombinant protein

Recombinant Proteins; Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 1004007314
• NCBI GeneID: 3716
• NCBI Accession #: NP_001307852.1
• NCBI GenBank Nucleotide #: NM_001320923.1
• UniProt Accession #: P23458; Q59GQ2; Q9UD26
• Molecular Weight: 133,277 Da
• NCBI Official Full Name: tyrosine-protein kinase JAK1 isoform 1
• NCBI Official Synonym Full Names: Janus kinase 1
• NCBI Official Symbol: JAK1
• NCBI Official Synonym Symbols: JTK3; JAK1A; JAK1B
• NCBI Protein Information: tyrosine-protein kinase JAK1
• UniProt Protein Name: Tyrosine-protein kinase JAK1
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: JAK1
• UniProt Synonym Gene Names: JAK1A; JAK1B; JAK-1
• UniProt Entry Name: JAK1_HUMAN

NCBI Description

This gene encodes a membrane protein that is a member of a class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The encoded kinase phosphorylates STAT proteins (signal transducers and activators of transcription) and plays a key role in interferon-alpha/beta and interferon-gamma signal transduction. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]

Uniprot Description

JAK1: a widely expressed non-receptor tyrosine-kinase involved in the interferon-alpha/beta and -gamma signal transduction pathways. Couples cytokine ligand binding to tyrosine phosphorylation of various known signaling proteins and of a unique family of transcription factors termed the signal transducers and activators of transcription, or STATs. A selective Jak1 inhibitor induces apoptosis in NRP-154 prostate cancer cell line. A single mutation seen in tyrosine kinome screen of colon cancers. Activated in B cell lines from patients with post-transplant lymphoproliferative disorder, and in a mouse Lck-driven T cell lymphoma. Inhibitor: Piceatannol.

Protein type: EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); Protein kinase, TK; Kinase, protein; TK group; JakA family

Chromosomal Location of Human Ortholog: 1p32.3-p31.3

Cellular Component: extrinsic to internal side of plasma membrane; cytoskeleton; focal adhesion; cytoplasm; endomembrane system; nucleus; cytosol

Molecular Function: protein binding; growth hormone receptor binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; protein phosphatase binding; ATP binding; CCR5 chemokine receptor binding

Biological Process: response to antibiotic; cell migration; cytokine and chemokine mediated signaling pathway; innate immune response; cell differentiation; protein amino acid phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of cell proliferation