STK11/LKB1 recombinant protein

Recombinant STK11/LKB1 protein

Cat. Num. AAA389423

• Gene Names: STK11; PJS; LKB1; hLKB1
• Synonyms: STK11/LKB1; Recombinant STK11/LKB1 protein; Serine/threonine kinase 11; STK11/LKB1 recombinant protein

• Host: Baculovirus
• Form/Format: Recombinant STK11/LKB1 protein is supplied in 25mM HEPES-NaOH pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100, 0.5mM TCEP.

• Protein Species: Human
• Tag: DYKDDDDK-Tag
• Protein Details: Recombinant STK11/LKB1 protein was expressed in a baculovirus expression system as the full length protein (accession number NP_000446.1) with an N-terminal DYKDDDDK tag.
• Notes: This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant STK11 / LKB1 protein 10% SDS-PAGEwith Coomassie staining MW: 49.6 kDa Purity: >80%)

Testing Data

(HTRF assay for STK11 / LKB1 activity 1 uM STK S1 substrate was incubated with different concentrations of STK11 / LKB1 protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, and 100u?M ATP for 1 hour. Then 10 ul detection reagents containing STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)

Related Product Information for STK11/LKB1 recombinant protein

Background: STK11 (Serine/Threonine Kinase 11) is a tumor suppressor serine/threonine-protein kinase that controls the activity of AMP-activated protein kinase (AMPK) family members and plays a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. STK11 phosphorylates the T-loop of AMPK family proteins, thus promoting their activity. STK11 phosphorylates PRKAA1, PRKAA2, BRSK1, BRSK2, MARK1, MARK2, MARK3, MARK4, NUAK1, NUAK2, SIK1, SIK2, SIK3 and SNRK but not MELK and also phosphorylates non-AMPK family proteins such as STRADA, PTEN and possibly p53/TP53. STK11 acts as a key upstream regulator of AMPK by mediating phosphorylation and activation of AMPK catalytic subunits PRKAA1 and PRKAA2 and thereby regulates processes including: inhibition of signaling pathways that promote cell growth and proliferation when energy levels are low, glucose homeostasis in liver, activation of autophagy when cells undergo nutrient deprivation, and B-cell differentiation in the germinal center in response to DNA damage.

Product Categories/Family for STK11/LKB1 recombinant protein

Recombinant Proteins; Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 4507271
• NCBI GeneID: 6794
• NCBI Accession #: NP_000446.1
• NCBI GenBank Nucleotide #: NM_000455.4
• UniProt Accession #: Q15831; B2RBX7; E7EW76
• Molecular Weight: 48,636 Da
• NCBI Official Full Name: serine/threonine-protein kinase STK11
• NCBI Official Synonym Full Names: serine/threonine kinase 11
• NCBI Official Symbol: STK11
• NCBI Official Synonym Symbols: PJS; LKB1; hLKB1
• NCBI Protein Information: serine/threonine-protein kinase STK11; liver kinase B1; polarization-related protein LKB1; renal carcinoma antigen NY-REN-19; serine/threonine-protein kinase 11; serine/threonine-protein kinase LKB1
• UniProt Protein Name: Serine/threonine-protein kinase STK11
• UniProt Gene Name: STK11
• UniProt Synonym Gene Names: LKB1; PJS; LKB1; hLKB1
• UniProt Entry Name: STK11_HUMAN

NCBI Description

This gene, which encodes a member of the serine/threonine kinase family, regulates cell polarity and functions as a tumor suppressor. Mutations in this gene have been associated with Peutz-Jeghers syndrome, an autosomal dominant disorder characterized by the growth of polyps in the gastrointestinal tract, pigmented macules on the skin and mouth, and other neoplasms. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

LKB1: a S/T protein kinase of the CAMKL family. A tumor suppressor that helps control cell structure, polarity, apoptosis and energy homeostasis. Phosphorylates AGS3 (activator of G-protein signaling 3) GPR domains, regulating the interaction of GPR-containing proteins with G-proteins. A cytosolic protein complex comprised of LKB1, the pseudokinase STRAD, and the MO25 scaffold protein. Activates AMPK and several related protein kinases. AMPK plays a predominant role as the master regulator of cellular energy homeostasis, controlling downstream effectors that regulate cell growth and apoptosis in response to cellular ATP concentrations. The Lkb1/Strad/Mo25 complex can polarize single epithelial cells, leading to the formation of a brush border containing microvilli on the apical surface. Mutation in the corresponding gene causes Peutz-Jeghers syndrome (PJS), an autosomal dominant disorder characterized by benign GI tract polyps and dark skin lesions of the mouth, hands and feet. A variety of other LKB1 gene mutations have been associated with the formation of sporadic cancers in several tissues.

Protein type: Protein kinase, CAMK; Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Autophagy; Tumor suppressor; CAMK group; CAMKL family; LKB subfamily

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: mitochondrion; membrane; cytoplasm; nucleus; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; p53 binding; magnesium ion binding; protein complex binding; protein kinase activator activity; ATP binding; LRR domain binding

Biological Process: Golgi localization; response to glucagon stimulus; protein heterooligomerization; protein amino acid autophosphorylation; Wnt receptor signaling pathway through beta-catenin; response to lipid; glucose homeostasis; T cell receptor signaling pathway; protein amino acid phosphorylation; anoikis; negative regulation of cell proliferation; positive regulation of gluconeogenesis; regulation of fatty acid biosynthetic process; vasculature development; tissue homeostasis; regulation of cell growth; cell cycle arrest; positive thymic T cell selection; regulation of Wnt receptor signaling pathway; positive regulation of transforming growth factor beta receptor signaling pathway; regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; axonogenesis; establishment of cell polarity; insulin receptor signaling pathway; activation of protein kinase activity; energy reserve metabolic process; autophagy; positive regulation of axonogenesis; negative regulation of cell growth; response to ionizing radiation; response to DNA damage stimulus; spermatid development

Disease: Pancreatic Cancer; Testicular Germ Cell Tumor; Peutz-jeghers Syndrome