LYN recombinant protein

Recombinant LYN protein

Cat. Num. AAA389411

• Gene Names: LYN; JTK8; p53Lyn; p56Lyn
• Synonyms: LYN; Recombinant LYN protein; LYN Proto-Oncogene; Src Family Tyrosine Kinase; JTK8; LYN recombinant protein

• Host: Baculovirus
• Form/Format: Recombinant LYN protein is supplied in 25mM HEPES-NaOH pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100, and 0.5mM TCEP.

• Protein Species: Human
• Tag: DYKDDDDK-Tag
• Protein Details: Recombinant LYN protein was expressed in baculovirus expression system as the full length protein (accession number NP_001104567.1) with a N-terminal DYKDDDDK tag.
• Notes: This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant LYN protein 10% SDS-PAGE gel with Coomassie blue staining MW: 57 kDa Purity: >90%)

Testing Data

(HTRF assay for LYN activity 1 uM TK substrate was incubated with different concentrations of LYN protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 5 nM SEB and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)

Related Product Information for LYN recombinant protein

Background: LYN (LYN Proto-Oncogene, Src Family Tyrosine Kinase) is a non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors and plays an important role in the regulation of innate and adaptive immune responses, hematopoiesis, responses to growth factors and cytokines, integrin signaling, but also responses to DNA damage and genotoxic agents. Functions primarily as negative regulator, but can also function as activator, depending on the context. LYN plays an important role in the regulation of B-cell differentiation, proliferation, survival and apoptosis, and is important for immune self-tolerance. Regulates cell proliferation, survival, differentiation, migration, adhesion, degranulation, and cytokine release. It down-regulates signaling pathways by phosphorylation of immunoreceptor tyrosine-based inhibitory motifs (ITIM), that then serve as binding sites for phosphatases, such as PTPN6/SHP-1, PTPN11/SHP-2 and INPP5D/SHIP-1, that modulate signaling by dephosphorylation of kinases and their substrates. LYN regulates activation of the MAP kinase signaling cascade, including activation of MAP2K1/MEK1, MAPK1/ERK2, MAPK3/ ERK1, MAPK8/JNK1 and MAPK9/JNK2. Diseases associated with LYN include Sarcoma and Mastocytosis, Cutaneous.

Product Categories/Family for LYN recombinant protein

Recombinant Proteins; Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 162287326
• NCBI GeneID: 4067
• NCBI Accession #: NP_001104567.1
• NCBI GenBank Nucleotide #: NM_001111097.2
• UniProt Accession #: P07948; A0AVQ5
• Molecular Weight: 512
• NCBI Official Full Name: tyrosine-protein kinase Lyn isoform B
• NCBI Official Synonym Full Names: v-yes-1 Yamaguchi sarcoma viral related oncogene homolog
• NCBI Official Symbol: LYN
• NCBI Official Synonym Symbols: JTK8; p53Lyn; p56Lyn
• NCBI Protein Information: tyrosine-protein kinase Lyn; lck/Yes-related novel protein tyrosine kinase
• UniProt Protein Name: Tyrosine-protein kinase Lyn
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: LYN
• UniProt Synonym Gene Names: JTK8
• UniProt Entry Name: LYN_HUMAN