Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Target of rapamycin complex 2 subunit MAPKAP1 (MAPKAP1) Standard Curve.)
Mouse Target Of Rapamycin Complex 2 Subunit MAPKAP1 (MAPKAP1) ELISA Kit | MAPKAP1 elisa kit
Mouse Target Of Rapamycin Complex 2 Subunit MAPKAP1 (MAPKAP1) ELISA Kit
Gene Names
Mapkap1; Sin1; AI591529; D230039K05Rik
Reactivity
Mouse
Synonyms
Target Of Rapamycin Complex 2 Subunit MAPKAP1 (MAPKAP1); Mouse Target Of Rapamycin Complex 2 Subunit MAPKAP1 (MAPKAP1) ELISA Kit; MAPKAP1; RP11-269P11.1; JC310; MGC2745; MIP1; SIN1; SIN1b; SIN1g; MEKK2-interacting protein 1; OTTHUMP00000022141; SAPK-interacting protein 1; ras inhibitor MGC2745; stress-activated map kinase interacting prote; MAPKAP1 elisa kit
Reactivity
Mouse
Specificity
Mouse Target of rapamycin complex 2 subunit MAPKAP1 (MAPKAP1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MAPKAP1. No significant cross-reactivity or interference between Mouse MAPKAP1 and analogues was observed.
Sequence Length
522
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Target of rapamycin complex 2 subunit MAPKAP1 (MAPKAP1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Target of rapamycin complex 2 subunit MAPKAP1 (MAPKAP1) Standard Curve.)
Related Product Information for MAPKAP1 elisa kit
Description: This Mouse Target of rapamycin complex 2 subunit MAPKAP1 (MAPKAP1) ELISA Kit employs a two-site sandwich ELISA to quantitate MAPKAP1 in samples. An antibody specific for MAPKAP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MAPKAP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAPKAP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAPKAP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Target of rapamycin complex 2 subunit MAPKAP1 is a protein similar to the yeast SIN1 protein, a stress-activated protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been described. Alternate polyadenylation sites as well as alternate 3' UTRs have been identified for transcripts of this gene. The deduced 522-amino acid protein has a nuclear localization signal, 2 bipartite nuclear localization signals, a peroxisomal targeting signal, and a PEST motif for rapid protein degradation. Comparison of SIN1 with homologs from various species revealed a short, highly conserved region, designated Box1, located within a larger conserved domain, designated CRIM (conserved region in middle).
Background: Target of rapamycin complex 2 subunit MAPKAP1 is a protein similar to the yeast SIN1 protein, a stress-activated protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been described. Alternate polyadenylation sites as well as alternate 3' UTRs have been identified for transcripts of this gene. The deduced 522-amino acid protein has a nuclear localization signal, 2 bipartite nuclear localization signals, a peroxisomal targeting signal, and a PEST motif for rapid protein degradation. Comparison of SIN1 with homologs from various species revealed a short, highly conserved region, designated Box1, located within a larger conserved domain, designated CRIM (conserved region in middle).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
target of rapamycin complex 2 subunit MAPKAP1 isoform a
NCBI Official Synonym Full Names
mitogen-activated protein kinase associated protein 1
NCBI Official Symbol
Mapkap1
NCBI Official Synonym Symbols
Sin1; AI591529; D230039K05Rik
NCBI Protein Information
target of rapamycin complex 2 subunit MAPKAP1
UniProt Protein Name
Target of rapamycin complex 2 subunit MAPKAP1
Protein Family
UniProt Gene Name
Mapkap1
UniProt Synonym Gene Names
Mip1; Sin1; TORC2 subunit MAPKAP1; SAPK-interacting protein 1
UniProt Entry Name
SIN1_MOUSE
Research Articles on MAPKAP1
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Product Notes
The Mouse MAPKAP1 mapkap1 (Catalog #AAA9717738) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717738 ELISA Kit recognizes Mouse MAPKAP1. It is sometimes possible for the material contained within the vial of "Target Of Rapamycin Complex 2 Subunit MAPKAP1 (MAPKAP1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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