SDS-Page
(Recombinant STK10 / LOK (1-348) protein 10% SDS-PAGE with Coomassie staining MW: 40.4 kDa Purity: >80%)
STK10/LOK recombinant protein
Recombinant STK10/LOK (1-348) protein
Shipping Temp: Dry Ice
SDS-Page
(Recombinant STK10 / LOK (1-348) protein 10% SDS-PAGE with Coomassie staining MW: 40.4 kDa Purity: >80%)
SDS-Page
(Recombinant STK10 / LOK (1-348) protein 10% SDS-PAGE with Coomassie staining MW: 40.4 kDa Purity: >80%)
Testing Data
(HTRF assay for STK10 / LOK (1-348) activity 1 uM STK S3 substrate was incubated with different concentrations of STK10 / LOK (1-348) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Testing Data
(HTRF assay for STK10 / LOK (1-348) activity 1 uM STK S3 substrate was incubated with different concentrations of STK10 / LOK (1-348) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the Ste20 family of serine/threonine protein kinases, and is similar to several known polo-like kinase kinases. The protein can associate with and phosphorylate polo-like kinase 1, and overexpression of a kinase-dead version of the protein interferes with normal cell cycle progression. The kinase can also negatively regulate interleukin 2 expression in T-cells via the mitogen activated protein kinase kinase 1 pathway. [provided by RefSeq, Jul 2008]
Uniprot Description
LOK: a protein kinase of the STE20 family. Highly expressed in hematopoietic tissue. May cooperate with SLK to regulate Plk1 function in human cells.
Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; Protein kinase, STE; STE group; STE20 family; SLK subfamily
Chromosomal Location of Human Ortholog: 5q35.1
Cellular Component: cytoplasm; plasma membrane
Molecular Function: protein serine/threonine kinase activity; identical protein binding; protein binding; polo kinase kinase activity; protein homodimerization activity; ATP binding
Biological Process: protein amino acid autophosphorylation; cell cycle; protein amino acid phosphorylation
Research Articles on STK10/LOK
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Product Notes
The STK10/LOK stk10 (Catalog #AAA389422) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "STK10/LOK, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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