SDS-Page
(Recombinant BTK protein gel 10% SDS-PAGE Coomassie staining MW: 77.6 kDa Purity: ? 90%)
BTK recombinant protein
Recombinant BTK protein
Shipping Temp: Dry Ice
SDS-Page
(Recombinant BTK protein gel 10% SDS-PAGE Coomassie staining MW: 77.6 kDa Purity: ? 90%)
SDS-Page
(Recombinant BTK protein gel 10% SDS-PAGE Coomassie staining MW: 77.6 kDa Purity: ? 90%)
Testing Data
(HTRF assay for BTK activity 1 uM TK substrate was incubated with different concentrations of BTK protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 5 nM SEB and 100 uM ATP for 1 hour. The detection reagents containing anti-TK antibody and SA-XL665, diluted in 1X Detection Buffer, were added and incubated with the reactions for 30 min. All operations and reactions were performed at room temperature, and HTRF KinASE TK assay was used to detect the enzymatic activity.)
Testing Data
(HTRF assay for BTK activity 1 uM TK substrate was incubated with different concentrations of BTK protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 5 nM SEB and 100 uM ATP for 1 hour. The detection reagents containing anti-TK antibody and SA-XL665, diluted in 1X Detection Buffer, were added and incubated with the reactions for 30 min. All operations and reactions were performed at room temperature, and HTRF KinASE TK assay was used to detect the enzymatic activity.)
Testing Data
(HTRF assay for BTK activity 1 uM TK substrate was incubated with different concentrations of BTK protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 5 nM SEB and 100 uM ATP for 1 hour. The detection reagents containing anti-TK antibody and SA-XL665, diluted in 1X Detection Buffer, were added and incubated with the reactions for 30 min. All operations and reactions were performed at room temperature, and HTRF KinASE TK assay was used to detect the enzymatic activity.)
Testing Data
(HTRF assay for BTK activity 1 uM TK substrate was incubated with different concentrations of BTK protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 5 nM SEB and 100 uM ATP for 1 hour. The detection reagents containing anti-TK antibody and SA-XL665, diluted in 1X Detection Buffer, were added and incubated with the reactions for 30 min. All operations and reactions were performed at room temperature, and HTRF KinASE TK assay was used to detect the enzymatic activity.)
Background: BTK (Bruton Tyrosine Kinase), also known as B-Cell Progenitor Kinase, EC 2.7.10.2, AGMX1, ATK, BPK, is a non-receptor tyrosine kinase indispensable for B lymphocyte development, differentiation and signaling. It acts as a platform to bring together a diverse array of signaling proteins and is implicated in cytokine receptor signaling pathways.. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. After BCR engagement and activation at the plasma membrane, BTK can phosphorylate PLCG2 at several sites, igniting the downstream signaling pathway through calcium mobilization, followed by activation of the protein kinase C (PKC) family members. It also plays an important role in the function of immune cells of innate as well as adaptive immunity, as a component of the Toll-like receptors (TLR) pathway. BTK can be activated by phosphorylation. In primary B lymphocytes, it is almost always non-phosphorylated and is thus catalytically inactive. Stimulation of TLR8 and TLR9 causes BTK activation. As a negative feedback mechanism to fine-tune BCR signaling, activated PRKCB down-modulates BTK function via direct phosphorylation of BTK at Ser-180, resulting in translocation of BTK back to the cytoplasmic fraction. PIN1, SH3BP5, and IBTK were also identified as BTK activity inhibitors. Interaction with CAV1 leads to dramatic down-regulation of the kinase activity of BTK. LFM-13A is a specific inhibitor of BTK.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement. [provided by RefSeq, Nov 2008]
Uniprot Description
Btk: a tyrosine kinase of the Tec family. Plays a crucial role in B-cell ontogeny. Defects cause X-linked agammaglobulinemia, an immunodeficiency characterized by failure to produce mature B lymphocyte cells and associated with a failure of Ig heavy chain rearrangement. Truncated splice forms found in childhood leukemias may underlie radiation resistance of tumors through inhibition of apoptosis.
Protein type: Protein kinase, TK; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); Kinase, protein; TK group; Tec family
Chromosomal Location of Human Ortholog: Xq21.33-q22
Cellular Component: extrinsic to internal side of plasma membrane; mast cell granule; intracellular membrane-bound organelle; perinuclear region of cytoplasm; cytoplasm; plasma membrane; cytoplasmic vesicle; nucleus; cytosol; lipid raft
Molecular Function: identical protein binding; protein binding; phosphatidylinositol-3,4,5-triphosphate binding; metal ion binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; ATP binding; receptor binding
Biological Process: I-kappaB kinase/NF-kappaB cascade; adaptive immune response; transcription, DNA-dependent; B cell activation; cell maturation; calcium-mediated signaling; protein amino acid phosphorylation; T cell receptor signaling pathway; regulation of cell proliferation; toll-like receptor 2 signaling pathway; activation of NF-kappaB transcription factor; regulation of B cell cytokine production; MyD88-dependent toll-like receptor signaling pathway; regulation of B cell apoptosis; B cell receptor signaling pathway; histamine secretion by mast cell; toll-like receptor signaling pathway; innate immune response; positive regulation of B cell differentiation; mesoderm development; cell differentiation; toll-like receptor 4 signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway
Disease: Isolated Growth Hormone Deficiency, Type Iii; Agammaglobulinemia, X-linked
Research Articles on BTK
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Product Notes
The BTK btk (Catalog #AAA389140) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "BTK, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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