CD7 elisa pair set

CD7 Matched ELISA Antibody Pair Set, Human

Cat. Num. AAA8118338

• Synonyms: CD7; CD7 Matched ELISA Antibody Pair Set; Human; Human CD7 ELISA Pair Set; CD7 molecule; GP40 Matched ELISA Antibody Pair Set; LEU-9 Matched ELISA Antibody Pair Set; Tp40 Matched ELISA Antibody Pair Set; TP41 Matched ELISA Antibody Pair Set; CD7 elisa pair set

• Specificity: Quantitative determination of Human CD7

• Assay Type: Solid Phase Sandwich ELISA
• Detection Range: 15.63-1000pg/mL
• Sensitivity: 15.625pg/ml
• Materials Provided: 1. Capture Antibody 0.5 mg/mL of rabbit anti-CD7 monoclonal antibody. Dilute to a working concentration of 2.0ug/mL in CBS before coating. ; 2. Detection Antibody 0.5 mg/mL rabbit anti-CD7 polyclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use.3. Standard Each vial contains 110 ng of recombinant CD7. Reconstitute standard powder with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1000 pg/mL is recommended.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20 degree C to -80 degree C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Testing Data

Related Product Information for CD7 elisa pair set

The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD7 is a transmembrane protein that is a member of the immunoglobulin superfamily. This protein is found on thymocytes and mature T cells. It plays an essential role in T-cell interactions and also in T-cell/B-cell interaction during early lymphoid development.

This CD7 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human CD7. It contains Human CD7 capture antibody, Human CD7 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human CD7 protein.

References

Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.