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Testing Data

DKK3 elisa pair set

DKK3 Matched ELISA Antibody Pair Set, Mouse

Synonyms
DKK3; DKK3 Matched ELISA Antibody Pair Set; Mouse; Mouse DKK3 ELISA Pair Set; dickkopf WNT signaling pathway inhibitor 3; AW061014 Matched ELISA Antibody Pair Set; C87148 Matched ELISA Antibody Pair Set; DKK3 elisa pair set
Ordering
For Research Use Only!
Specificity
Quantitative determination of Mouse DKK3
Assay Type
Solid Phase Sandwich ELISA
Detection Range
15.63-1000pg/mL
Sensitivity
15.625pg/ml
Materials Provided
1. Capture Antibody 0.5 mg/mL of rabbit anti-mouse DKK3 monoclonal antibody. Dilute to a working concentration of 2.0ug/mL in CBS before coating.
2. Detection Antibody 0.5 mg/mL rabbit anti-mouse DKK3 polyclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.25ug/mL in detection antibody dilution buffer before use.3. Standard Each vial contains 15 ng of recombinant mouse DKK3. Reconstitute standard powder with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1000 pg/mL is recommended.
Preparation and Storage
Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20 degree C to -80 degree C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Testing Data

Testing Data
Related Product Information for DKK3 elisa pair set
DKK3 (dickkopf related protein 3) is a member of the dickkopf-related family consisting of DKK1, DKK2, DKK3 and DKK4. It is a secreted protein, and also known as REIC (Reduced Expansion in Immortalized Cells). The DKK3 protein is proposed to function as a secreted tumor suppressor since it is downregulated in a number of cancer cells and prostate cancer tissue and may be a promising candidate molecule for therapeutic interference. DKK3 protein is also a negative regulator of beta-catenin and its downregulation contribute to an activation of the beta-catenin signaling pathway.

This DKK3 Matched ELISA Antibody Pair Set, Mouse is a solid phase sandwich ELISA for quantitative determination of Mouse DKK3. It contains Mouse DKK3 capture antibody, Mouse DKK3 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Mouse DKK3 protein.
Product Categories/Family for DKK3 elisa pair set
References
Yue W, et al., 2008. Downregulation of Dkk3 activates beta-catenin/TCF-4 signaling in lung cancer. Carcinogenesis, 29 (1): 84-92.Ding Z, et al., 2009. Promoter methylation and mRNA expression of DKK3 and WIF-1 in hepatocellular carcinoma. World J Gastroenterol, 15 (21):2595-601.

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Product Notes

The DKK3 (Catalog #AAA8118484) is an ELISA Pair Set and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "DKK3, ELISA Pair Set" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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