SIGNR1 ELISA Pair Set | CD209B elisa pair set

SIGNR1 Matched ELISA Antibody Pair Set, Mouse

Cat. Num. AAA8118506

• Synonyms: SIGNR1; SIGNR1 Matched ELISA Antibody Pair Set; Mouse; Mouse SIGNR1/CD209b ELISA Pair Set; CD209b antigen; 1810030I22Rik Matched ELISA Antibody Pair Set; DC-SIGNR1 Matched ELISA Antibody Pair Set; mSIGNR1 Matched ELISA Antibody Pair Set; OtB7 Matched ELISA Antibody Pair Set; CD209B elisa pair set

• Specificity: Quantitative determination of Mouse SIGNR1

• Assay Type: Solid Phase Sandwich ELISA
• Detection Range: 6.25-400pg/mL
• Sensitivity: 6.25pg/ml
• Materials Provided: 1. Capture Antibody 0.4 mg/mL of rabbit anti-mouse CD209B monoclonal antibody. Dilute to a working concentration of 2ug/mL in CBS before coating. ; 2. Detection Antibody 0.25 mg/mL rabbit anti-mouse CD209B monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use. ; 3. Standard Each vial contains 16 ng of recombinant mouse CD209B. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 0.4ng/mL is recommended.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20 degree C to -80 degree C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Testing Data

Related Product Information for CD209B elisa pair set

The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD209b, also known as SIGNR1, is a C-type lectin receptor. CD209b is present on most regions of mouse brain and found on microglia and dendritic cells but not on neurons or astrocytes. CD209b is implicated in the recently described SIGNR1 complement activation pathway, which operates against capsular polysaccharides in splenic marginal macrophages. CD209b in rat is homologue to SIGNR1 in mouse, both of which are found to mediate the uptake of dextran or CPS14 within the splenic marginal zone.

This SIGNR1 Matched ELISA Antibody Pair Set, Mouse is a solid phase sandwich ELISA for quantitative determination of Mouse SIGNR1. It contains Mouse SIGNR1 capture antibody, Mouse SIGNR1 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Mouse SIGNR1 protein.

References

Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.Park JY, et al. (2009) The C-type lectin CD209b is expressed on microglia and it mediates the uptake of capsular polysaccharides of Streptococcus pneumonia. Neuroscience Letters. 450 (3): 246-51.