Rabbit anti-Human RBBP8 Polyclonal Antibody | anti-RBBP8 antibody

RBBP8, CT (RBBP8, CTIP, DNA endonuclease RBBP8, CtBP-interacting protein, Retinoblastoma-binding protein 8, Retinoblastoma-interacting protein and myosin-like, Sporulation in the absence of SPO11 protein 2 homolog) (PE)

Cat. Num. AAA6340629

• Gene Names: RBBP8; RIM; COM1; CTIP; JWDS; SAE2; SCKL2
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RBBP8; Polyclonal Antibody; CT (RBBP8; CTIP; DNA endonuclease RBBP8; CtBP-interacting protein; Retinoblastoma-binding protein 8; Retinoblastoma-interacting protein and myosin-like; Sporulation in the absence of SPO11 protein 2 homolog) (PE); Sporulation in the absence of SPO11 protein 2 homolog; anti-RBBP8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Sequence Length: 867

• Applicable Applications for anti-RBBP8 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RBBP8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 762-792 amino acids from the C-terminal region of human RBBP8.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(RBBP8 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the RBBP8 antibody detected the RBBP8 protein (arrow).)

Related Product Information for anti-RBBP8 antibody

The protein encoded by this gene is a ubiquitously expressed nuclear protein. It is found among several proteins that bind directly to retinoblastoma protein, which regulates cell proliferation. This protein complexes with transcriptional co-repressor CTBP. It is also associated with BRCA1 and is thought to modulate the functions of BRCA1 in transcriptional regulation, DNA repair, and/or cell cycle checkpoint control. It is suggested that this gene may itself be a tumor suppressor acting in the same pathway as BRCA1. Three transcript variants encoding two different isoforms have been found for this gene. More transcript variants exist, but their full-length natures have not been determined.

Product Categories/Family for anti-RBBP8 antibody

Antibodies; Retinoblastoma Proteins

References

Kaidi, A., et al. Science 329(5997):1348-1353(2010). Thye, T., et al. Nat. Genet. 42(9):739-741(2010). Notaridou, M., et al. Int. J. Cancer (2010). In press: Yasuno, K., et al. Nat. Genet. 42(5):420-425(2010). Zhao, J., et al. BMC Med. Genet. 11, 96 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 42718017
• NCBI GeneID: 5932
• NCBI Accession #: NP_976037.1; NP_976036.1; NP_002885.1
• NCBI GenBank Nucleotide #: NP_976037.1
• UniProt Accession #: Q99708
• NCBI Official Full Name: DNA endonuclease RBBP8 isoform b
• NCBI Official Synonym Full Names: RB binding protein 8, endonuclease
• NCBI Official Symbol: RBBP8
• NCBI Official Synonym Symbols: RIM; COM1; CTIP; JWDS; SAE2; SCKL2
• NCBI Protein Information: DNA endonuclease RBBP8
• UniProt Protein Name: DNA endonuclease RBBP8
• Protein Family: RBBP8 N-terminal-like protein
• UniProt Gene Name: RBBP8
• UniProt Synonym Gene Names: CTIP; CtIP; RBBP-8; RIM; SAE2
• UniProt Entry Name: COM1_HUMAN

NCBI Description

The protein encoded by this gene is a ubiquitously expressed nuclear protein. It is found among several proteins that bind directly to retinoblastoma protein, which regulates cell proliferation. This protein complexes with transcriptional co-repressor CTBP. It is also associated with BRCA1 and is thought to modulate the functions of BRCA1 in transcriptional regulation, DNA repair, and/or cell cycle checkpoint control. It is suggested that this gene may itself be a tumor suppressor acting in the same pathway as BRCA1. Three transcript variants encoding two different isoforms have been found for this gene. More transcript variants exist, but their full-length natures have not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

CtIP: a transcription factor containing C2H2 zinc fingers. May modulate the functions ascribed to BRCA1 in transcriptional regulation, DNA repair, and/or cell cycle checkpoint control. Interacts with CTBP, with the C-terminal (BRCT) domains of BRCA1, and with the retinoblastoma protein. Abundantly expressed in brain and the immune system and associated with immune system malignancies. Ionizing radiation induces its hyperphosphorylation and dissociation from BRCA1 in an ATM-dependent manner.

Protein type: Transcription, coactivator/corepressor; EC 3.1.-.-

Chromosomal Location of Human Ortholog: 18q11.2

Cellular Component: nucleoplasm; transcriptional repressor complex; nucleolus; nucleus

Molecular Function: protein binding; single-stranded DNA specific endodeoxyribonuclease activity; damaged DNA binding

Biological Process: regulation of transcription from RNA polymerase II promoter; mitosis; meiotic cell cycle; cell division; blastocyst hatching; negative regulation of transcription from RNA polymerase II promoter; DNA repair; G2/M transition DNA damage checkpoint; cell cycle checkpoint; DNA catabolic process, endonucleolytic; double-strand break repair via homologous recombination; G1/S transition of mitotic cell cycle

Disease: Jawad Syndrome; Seckel Syndrome 2