Testing Data
CD89 ELISA Pair Set | FCAR elisa pair set
CD89 Matched ELISA Antibody Pair Set, Human
Synonyms
CD89; CD89 Matched ELISA Antibody Pair Set; Human; Human CD89/FCAR ELISA Pair Set; Fc fragment of IgA receptor; CTB-61M7.2 Matched ELISA Antibody Pair Set; FcalphaRI Matched ELISA Antibody Pair Set; FCAR Matched ELISA Antibody Pair Set; XXbac-BPG230H20.5 Matched ELISA Antibody Pair Set; FCAR elisa pair set
Specificity
Quantitative determination of Human CD89
Assay Type
Solid Phase Sandwich ELISA
Detection Range
7.81-500pg/mL
Sensitivity
7.8125pg/ml
Materials Provided
1. Capture Antibody 0.5 mg/mL of rabbit anti-CD89 monoclonal antibody, Dilute to a working concentration of 2.0ug/mL in CBS before coating.
2. Detection Antibody 0.25 mg/mL rabbit anti-CD89 polyclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1.0ug/mL in detection antibody dilution buffer before use.3. Standard Each vial contains 30 ng of recombinant CD89. Reconstitute standard powder with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 500 pg/mL is recommended.
2. Detection Antibody 0.25 mg/mL rabbit anti-CD89 polyclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1.0ug/mL in detection antibody dilution buffer before use.3. Standard Each vial contains 30 ng of recombinant CD89. Reconstitute standard powder with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 500 pg/mL is recommended.
Preparation and Storage
Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20 degree C to -80 degree C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20 degree C to -80 degree C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Testing Data
Testing Data
Related Product Information for FCAR elisa pair set
FCAR, also called FcalphaRI or CD89, is a type I transmembrane receptor for Fc region of IgA which is the most abundant immunoglobulin in mucosal areas but is only the second most common antibody isotype in serum. This receptor is present on the surface of myeloid lineage cells such as neutrophils, monocytes, macrophages, and eosinophils, especially phagocytes located in mucosal areas. Upon ligand IgA binding, FcalphaRI associates with the FcR gamma signaling molecule bearing the immunoreceptor tyrosine-based activation motif (ITAM) through a unique charge-based mechanism and triggers multiple cell-mediated immune responses. It has been reported that Fc RI is a dual-function receptor that can mediate both inflammatory and anti-inflammatory responses depending on the type of interaction with its ligand. Sustained aggregation of FCAR results in activation of target-cell functions such as antigen presentation and cytokine release. In contrast, Monomeric targeting with serum IgA or with a variety of anti-FcalphaRI Fab fragments triggers an inhibitory response and additionally induces apoptosis. FcalphaRI thus play an fundamental role in preventing tumor development and growth, as well as in controlling inflammation.
This CD89 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human CD89. It contains Human CD89 capture antibody, Human CD89 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human CD89 protein.
This CD89 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human CD89. It contains Human CD89 capture antibody, Human CD89 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human CD89 protein.
Product Categories/Family for FCAR elisa pair set
References
Maliszewski, C.R. et al., 1990, J. Exp. Med. 172: 1665-1672.
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Product Notes
The FCAR (Catalog #AAA8118247) is an ELISA Pair Set and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "CD89, ELISA Pair Set" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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