Testing Data
CD14 elisa pair set
CD14 Matched ELISA Antibody Pair Set, Mouse
Synonyms
CD14; CD14 Matched ELISA Antibody Pair Set; Mouse; Mouse CD14 ELISA Pair Set; CD14 molecule; CD14 elisa pair set
Specificity
Quantitative determination of Mouse CD14
Assay Type
Solid Phase Sandwich ELISA
Detection Range
78.13-5000pg/mL
Sensitivity
78.1pg/ml
Materials Provided
1. Capture Antibody 0.5 mg/mL of mouse anti-mouse CD14 monoclonal antibody. Dilute to a working concentration of 2ug/mL in CBS before coating.
2. Detection Antibody 0.5 mg/mL rabbit anti-mouse CD14 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1ug/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 90 ng of recombinant mouse CD14. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 5ng/mL is recommended.
2. Detection Antibody 0.5 mg/mL rabbit anti-mouse CD14 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1ug/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 90 ng of recombinant mouse CD14. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 5ng/mL is recommended.
Preparation and Storage
Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20 degree C to -80 degree C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20 degree C to -80 degree C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Testing Data
Testing Data
Related Product Information for CD14 elisa pair set
The cluster of differentiation (CD) system is commonly used as cell markers in immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 14 (CD14) is a member of the CD system. It takes its name from its inclusion in the CD molecule surface marker proteins. CD14 exists in two forms: a form anchored into the membrane or a soluble form. CD14 was found expressed in macrophages, neutrophil granulocyte and dendritic cells. The major function is to serve as a co-receptor (along with TLR4 and MD-2) for the bacterial lipopolysaccharide (LPS) and other pathogen-associated molecular patterns.
This CD14 Matched ELISA Antibody Pair Set, Mouse is a solid phase sandwich ELISA for quantitative determination of Mouse CD14. It contains Mouse CD14 capture antibody, Mouse CD14 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Mouse CD14 protein.
This CD14 Matched ELISA Antibody Pair Set, Mouse is a solid phase sandwich ELISA for quantitative determination of Mouse CD14. It contains Mouse CD14 capture antibody, Mouse CD14 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Mouse CD14 protein.
Product Categories/Family for CD14 elisa pair set
References
Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.SD Wright, et al. (1990) CD14, a receptor for complexes of lipopolysaccharide (LPS) and LPS binding protein. Science. 249 (4975): 1431-3.
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Product Notes
The CD14 (Catalog #AAA8118464) is an ELISA Pair Set and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "CD14, ELISA Pair Set" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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