Testing Data
Semaphorin 4D ELISA Pair Set | SEMA4D elisa pair set
Semaphorin 4D Matched ELISA Antibody Pair Set, Mouse
Synonyms
Semaphorin 4D; Semaphorin 4D Matched ELISA Antibody Pair Set; Mouse; Mouse Semaphorin 4D/SEMA4D/CD100 ELISA Pair Set; semaphorin 4D; CD100 Matched ELISA Antibody Pair Set; coll-4 Matched ELISA Antibody Pair Set; Semacl2 Matched ELISA Antibody Pair Set; Semaj Matched ELISA Antibody Pair Set; Semcl2 Matched ELISA Antibody Pair Set; SEMA4D elisa pair set
Specificity
Quantitative determination of Mouse Semaphorin 4D
Assay Type
Solid Phase Sandwich ELISA
Detection Range
46.88-3000pg/mL
Sensitivity
47pg/ml
Materials Provided
1. Capture Antibody 1.0 mg/mL of rabbit anti-mouse SEMA4D monoclonal antibody. Dilute to a working concentration of 2ug/mL in CBS before coating.
2. Detection Antibody 0.5 mg/mL rabbit anti-mouse SEMA4D monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 150 ng of recombinant mouse SEMA4D. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 3ng/mL is recommended.
2. Detection Antibody 0.5 mg/mL rabbit anti-mouse SEMA4D monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 150 ng of recombinant mouse SEMA4D. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 3ng/mL is recommended.
Preparation and Storage
Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20 degree C to -80 degree C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20 degree C to -80 degree C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Testing Data
Testing Data
Related Product Information for SEMA4D elisa pair set
Semaphorin 4D (SEMA4D or CD100) is a member of the semaphorin family of proteins and an important mediator of the movement and differentiation of multiple cell types, including those of the immune, vascular, and nervous systems. VEGF and SEMA4D had a positive correlation with the malignant degree of ovarian cancer, and SEMA4D can serve as an independent prognostic factor. SEMA4D was the first semaphorin described to have immune functions and serves important roles in T cell priming, antibody production, and cell-to-cell adhesion. Proteolytic cleavage of SEMA4D from the cell surface gives rise to a soluble fragment of SEMA4D (sSEMA4D). Similar to the transmembranal form, sSEMA4D is thought to have immunoregulatory properties.
This Semaphorin 4D Matched ELISA Antibody Pair Set, Mouse is a solid phase sandwich ELISA for quantitative determination of Mouse Semaphorin 4D. It contains Mouse Semaphorin 4D capture antibody, Mouse Semaphorin 4D detector antibody (HRP) and a highly purified HEK293-expressed recombinant Mouse Semaphorin 4D protein.
This Semaphorin 4D Matched ELISA Antibody Pair Set, Mouse is a solid phase sandwich ELISA for quantitative determination of Mouse Semaphorin 4D. It contains Mouse Semaphorin 4D capture antibody, Mouse Semaphorin 4D detector antibody (HRP) and a highly purified HEK293-expressed recombinant Mouse Semaphorin 4D protein.
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Product Notes
The SEMA4D (Catalog #AAA8118511) is an ELISA Pair Set and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Semaphorin 4D, ELISA Pair Set" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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