Tie-1 ELISA Pair Set | Tie1 elisa pair set

Tie-1 Matched ELISA Antibody Pair Set, Human

Cat. Num. AAA8118265

• Synonyms: Tie-1; Tie-1 Matched ELISA Antibody Pair Set; Human; Human Tie1 ELISA Pair Set; tyrosine kinase with immunoglobulin-like and EGF-like domains 1; JTK14 Matched ELISA Antibody Pair Set; TIE Matched ELISA Antibody Pair Set; Tie1 elisa pair set

• Specificity: Quantitative determination of Human Tie-1

• Assay Type: Solid Phase Sandwich ELISA
• Detection Range: 78.13-5000pg/mL
• Sensitivity: 78pg/ml
• Materials Provided: 1. Capture Antibody 0.5 mg/mL of mouse anti-Tie1 monoclonal antibody, Dilute to a working concentration of 2ug/mL in CBS before coating. ; 2. Detection Antibody 0.5 mg/mL mouse anti-Tie1 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use. ; 3. Standard Each vial contains 160 ng of recombinant Tie1. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 5ng/mL is recommended.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20 degree C to -80 degree C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Testing Data

Related Product Information for Tie1 elisa pair set

Tyrosine kinase with immunoglobulin-like and EGF-like domains 1 also known as Tie1 is an angiopoietin receptor and is an orphan receptor tyrosine kinase that is expressed almost exclusively in endothelial cells and that is required for normal embryonic vascular development. The receptor tyrosine kinase Tie1 is expressed primarily in vascular endothelial cells. The receptor has also been detected in epithelial tumours in breast, thyroid and gastric cancers and in tumour cell lines where it appears as a 45 kDa truncated receptor fragment. Tie1 promotes endothelial cell survival, but other studies have suggested that the Tie1 kinase has little to no activity. Embryos deficient in Tie1 failed to establish structural integrity of vascular endothelial cells, resulting in oedema and subsequently localized haemorrhage. Tie1 is significantly higher in human aortic endothelial cells than in human umbilical vein endothelial cells. Additionally, attachment of cells of monocytic lineage to endothelial cells is also enhanced by Tie1 expression. Collectively Tie1 has a proinflammatory property and may play a role in endothelial inflammatory diseases such as atherosclerosis.

This Tie-1 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human Tie-1. It contains Human Tie-1 capture antibody, Human Tie-1 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human Tie-1 protein.

Product Categories/Family for Tie1 elisa pair set

Receptor Tyrosine Kinases (RTKs)

References

Chan B, et al. (2008) Receptor tyrosine kinase Tie-1 overexpression in endothelial cells upregulates adhesion molecules. Biochem Biophys Res Commun. 371(3): 475-9.Sato TN, et al. (1995) Distinct roles of the receptor tyrosine kinases Tie-1 and Tie-2 in blood vessel formation. Nature. 376(6535): 70-4.Rees KA, et al. (2007) The receptor tyrosine kinase Tie1 is expressed and activated in epithelial tumour cell lines. Int J Oncol. 31(4): 893-7.Kontos CD, et al. (2002) The endothelial receptor tyrosine kinase Tie1 activates phosphatidylinositol 3-kinase and Akt to inhibit apoptosis. Mol Cell Biol. 22(6): 1704-13.