Mouse PDE6G Monoclonal Antibody | anti-PDE6G antibody

PDE6G antibody

Cat. Num. AAA834519

• Gene Names: PDE6G; PDEG; RP57
• Applications: Western Blot
• Purity: PDE6G antibody was purified by affinity chromatography.
• Synonyms: PDE6G; Monoclonal Antibody; PDE6G antibody; Monoclonal PDE6G; Anti-PDE6G; Phosphodiesterase 6G cGMP-specific rod gamma; PDEG; GMP-PDE gamma; RP57; DKFZp686C0587; PDEG 6; PDEG-6; anti-PDE6G antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 1H7
• Purity/Purification: PDE6G antibody was purified by affinity chromatography.
• Form/Format: Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 500 ug-1 mg/ml (varies by lot)
• Sequence Length: 87

• Applicable Applications for anti-PDE6G antibody: Western Blot (WB)
• Application Notes: WB: 1:2000
• Biological Significance: PDE6G encodes the gamma subunit of cyclic GMP-phosphodiesterase, which is composed of alpha- and beta- catalytic subunits and two identical, inhibitory gamma subunits. This gene is expressed in rod photoreceptors and functions in the phototransduction signaling cascade. It is also expressed in a variety of other tissues, and has been shown toregulate the c-Src protein kinase and G-protein-coupled receptor kinase 2.
• Cross-Reactivity: Human
• Immunogen: PDE6G antibody was raised in mouse using a full length recombinant protein of human PDE6G (NP_002593) produced in HEK293T cells, as the immunogen.
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PDE6G protein (Right) or empty vector (Left) detected with PDE6G antibody)

Related Product Information for anti-PDE6G antibody

Mouse monoclonal PDE6G antibody

Product Categories/Family for anti-PDE6G antibody

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 4505673
• NCBI GeneID: 5148
• NCBI Accession #: NP_002593
• NCBI GenBank Nucleotide #: NM_002602.3
• UniProt Accession #: P18545; Q3KP63; Q7Z3U8
• Molecular Weight: 9,643 Da
• NCBI Official Full Name: retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma
• NCBI Official Synonym Full Names: phosphodiesterase 6G, cGMP-specific, rod, gamma
• NCBI Official Symbol: PDE6G
• NCBI Official Synonym Symbols: PDEG; RP57
• NCBI Protein Information: retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma
• UniProt Protein Name: Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma
• Protein Family: Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase
• UniProt Gene Name: PDE6G
• UniProt Synonym Gene Names: PDEG; GMP-PDE gamma
• UniProt Entry Name: CNRG_HUMAN

NCBI Description

This gene encodes the gamma subunit of cyclic GMP-phosphodiesterase, which is composed of alpha- and beta- catalytic subunits and two identical, inhibitory gamma subunits. This gene is expressed in rod photoreceptors and functions in the phototransduction signaling cascade. It is also expressed in a variety of other tissues, and has been shown to regulate the c-Src protein kinase and G-protein-coupled receptor kinase 2. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2009]

Uniprot Description

PDE6G: inhibitory gamma subunit of the retinal photoreceptor type 6 cGMP phosphodiesterase (PDE-gamma). Phosphorylated by GRK2. Regulates EGFR dependent stimulation of ERK1/2. Forms a cellular complex with src. cGMP-PDEs are the effector molecules in G-protein-mediated phototransduction in vertebrate rods and cones. Participates in processes of transmission and amplification of the visual signal.

Protein type: Phosphodiesterase; Nucleotide Metabolism - purine; EC 3.1.4.35

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: plasma membrane

Molecular Function: enzyme inhibitor activity; 3',5'-cyclic-GMP phosphodiesterase activity; protein binding; spectrin binding; cGMP binding

Biological Process: rhodopsin mediated signaling; phototransduction, visible light; regulation of rhodopsin mediated signaling; visual perception; metabolic process; activation of MAPK activity; positive regulation of epidermal growth factor receptor signaling pathway; positive regulation of G-protein coupled receptor protein signaling pathway; negative regulation of catalytic activity

Disease: Retinitis Pigmentosa 57; Retinitis Pigmentosa